Physical maps and virulence of Anticarsia gemmatalis nucleopolyhedrovirus genomic variants

• Published in: Archives of virology Vol. 144; no. 10; pp. 1991 - 2006
• Main Authors: Maruniak, J.E, Garcia-Maruniak, A, Souza, M.L, Zanotto, P.M.A, Moscardi, F
• Format: Journal Article
• Language: English
• Published: Wien Springer 01-01-1999; New York, NY Springer Nature B.V
• Subjects: Animals; Anticarsia gemmatalis; Anticarsia gemmatalis multiple nucleopolyhedrovirus; Biological and medical sciences; biological control agents; Cell Line; cell lines; Fundamental and applied biological sciences. Psychology; Genetic Variation; Genetics; Genome, Viral; genomics; Lepidoptera - virology; Microbiology; Noctuidae; Nuclear polyhedrosis virus; Nucleopolyhedrovirus; Nucleopolyhedrovirus - genetics; Nucleopolyhedrovirus - pathogenicity; Pest Control, Biological; Physical Chromosome Mapping; restriction fragment length polymorphism; Restriction Mapping; Spodoptera; Spodoptera frugiperda; strain differences; Viral Plaque Assay; Virology; Virulence; South America; Brazil; America; Genetic mapping; Nucleopolyhedrovirus; Entomopathogen; Genome organization; Genetic variability; Insecta; Biological control; Anticarsia gemmatalis; Baculovirus; Characterization; Pathogenicity; Pest; Baculoviridae; Arthropoda; Lepidoptera; Noctuidae; Invertebrata
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s007050050720

Seventeen plaque purified isolates of two viral preparations of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), were analyzed in terms of the genomic changes after digestion of their DNAs with HindIII and PstI restriction enzymes. The 1979 AgMNPV wild type preparation (AgMNPV-'79) resulted in six different variants and the 1985 viral commercial preparation (AgMNPV-'85), in eleven. The genomic variation of all the isolates was mapped showing that those from 1985 presented more heterogeneity with changes mapped in additional sites in comparison to the AgMNPV-'79 variants. Their virulence was compared by infecting two Lepidopteran cell lines, Spodoptera frugiperda (IPLB-SF-21AE) and Anticarsia gemmatalis (UFL-AG-286). The results indicated that there was some difference in virulence within the AgMNPV-'85 variants. This commercial preparation had been applied in soybean fields in Brazil over several years to control the velvetbean caterpillar defoliation.