Total Th1/Th2 cytokines profile from peripheral blood lymphocytes in normal pregnancy and preeclampsia syndrome

• Published in: Hypertension in pregnancy Vol. 41; no. 1; pp. 15 - 22
• Main Authors: dos Santos Fagundes, Iara, Brendler, Eduardo Pletsch, Nunes Erthal, Isadora, Eder Ribeiro, Raquel Jaqueline, Caron-Lienert, Rafaela Siviero, Machado, Denise Cantarelli, Pinheiro da Costa, Bartira Ercília, Poli-de-Figueiredo, Carlos Eduardo
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02-01-2022; Taylor & Francis Group
• Subjects: Cytokines; Female; flow cytometry; Humans; inflammation; interleukins; Pre-Eclampsia; Pregnancy; Pregnancy induced hypertension; T-Lymphocytes, Helper-Inducer; Th1 Cells; Th2 Cells; flow cytometry; Pregnancy induced hypertension; inflammation; interleukins
• ISSN: 1064-1955; 1525-6065
• DOI: 10.1080/10641955.2021.2008424

To evaluate total Th1/Th2 cytokines in CD3
+
cells (immunocompetent T-lymphocytes) and peripheral blood lymphocytes, mostly CD4+ (T helper cells) and CD8+ (T-cytotoxic cells) subpopulations in preeclampsia. Total blood leukocytes and lymphocytes counts, percent cells: CD3
+
, INF-g
+
/CD3
+
, IL-4
+
/CD3
+
, and IL-10
+
/CD3
+
, CD4
+
/CD8
+
were determined by flow-cytometry. Preeclampsia (n= 26) and normal pregnancy (n= 25) participants were age and gestational age matched. CD4
+
lymphocytes count was higher in preeclampsia, compared with normal pregnancy (43.6 ± 5.8 vs 37.6 ± 5.6%; P< 0.001). CD3
+
cells Th1/Th2 shift was not detected in preeclampsia, yet may be present in other cell types, such as CD4
+
and CD3 - lymphocytes.