Diphenyl diselenide protects endothelial cells against oxidized low density lipoprotein-induced injury: Involvement of mitochondrial function

• Published in: Biochimie Vol. 105; pp. 172 - 181
• Main Authors: Hort, Mariana Appel, Straliotto, Marcos Raniel, de Oliveira, Jade, Amoêdo, Nívea Dias, da Rocha, João Batista Teixeira, Galina, Antônio, Ribeiro-do-Valle, Rosa Maria, de Bem, Andreza Fabro
• Format: Journal Article
• Language: English
• Published: France Elsevier B.V 01-10-2014
• Subjects: Animals; Apoptosis - drug effects; Atherosclerosis - drug therapy; Atherosclerosis - etiology; Atherosclerosis - metabolism; Benzene Derivatives - administration & dosage; Cattle; Cell Survival - drug effects; Diphenyl diselenide; Endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - pathology; Glutathione - metabolism; Humans; Lipoproteins, LDL - metabolism; Lipoproteins, LDL - toxicity; Mitochondria; Mitochondria - drug effects; Organoselenium Compounds - administration & dosage; Oxidative stress; Oxidative Stress - drug effects; Oxidized LDL; Protective Agents - administration & dosage; Protective Agents - metabolism; Reactive Oxygen Species - metabolism; Diphenyl diselenide; Oxidative stress; Mitochondria; Oxidized LDL; Endothelial cells
• ISSN: 0300-9084; 1638-6183
• DOI: 10.1016/j.biochi.2014.07.004

Elevated levels of oxidized low density lipoprotein (oxLDL) are considered to be one of the major risk factors for atherosclerosis and cardiovascular morbidity. The early stages of atherosclerosis are initiated by the accumulation of oxLDL and the induction of toxic effects on endothelial cells, resulting in endothelial dysfunction. The aim of this study was to investigate how diphenyl diselenide (DD), an organoselenium compound, protect vascular endothelial cells against the toxic effects of oxLDL in vitro. Our data showed that the treatment of bovine endothelial aortic cells (BAEC) with DD (0.1–1 μM) for 24 h protected from oxLDL-induced reactive species (RS) production and reduced glutathione (GSH) depletion. Moreover, DD (1 μM) per se improved the maximal mitochondrial respiratory capacity and prevented oxLDL-induced mitochondrial damage. In addition, DD could prevent apoptosis induced by oxLDL in BAEC. Results from this study may provide insight into a possible molecular mechanism underlying DD suppression of oxLDL-mediated vascular endothelial dysfunction.
•Diphenyl diselenide protects endothelial cells from oxLDL-induced toxicity.•Diphenyl diselenide improves mitochondrial function in endothelial cells.•OxLDL-induced oxidative stress is prevented by Diphenyl diselenide.