Epidermal growth factor and its receptors in human pancreatic carcinoma

• Published in: Pancreas Vol. 5; no. 3; pp. 278 - 283
• Main Authors: YUAN FANG CHEN, GUO-ZONG PAN, XUE HOU, TONG-HUA LIU, JIE CHEN, YANAIHARA, C, YANAIHARA, N
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 01-05-1990
• Subjects: 550901 - Pathology- Tracer Techniques; ANIMAL TISSUES; ANIMALS; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BIOASSAY; BIOCHEMICAL REACTION KINETICS; Biological and medical sciences; BIOLOGICAL FUNCTIONS; BODY; CARCINOMAS; CELL TRANSFORMATIONS; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; Epidermal Growth Factor - metabolism; Epidermal Growth Factor - physiology; EPIDERMIS; EPITHELIUM; ErbB Receptors - metabolism; ErbB Receptors - physiology; FUNCTIONS; Gastroenterology. Liver. Pancreas. Abdomen; GLANDS; GROWTH FACTORS; Humans; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; IODINE 125; IODINE ISOTOPES; Iodine Radioisotopes; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; Liver. Biliary tract. Portal circulation. Exocrine pancreas; MAMMALS; MAN; Medical sciences; MEMBRANE PROTEINS; MITOGENS; NEOPLASMS; NUCLEI; ODD-EVEN NUCLEI; ONCOGENIC TRANSFORMATIONS; ORGANIC COMPOUNDS; ORGANS; PANCREAS; Pancreatic Neoplasms - metabolism; PRIMATES; PROTEINS; RADIOASSAY; RADIOIMMUNOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; RADIOISOTOPES; Radioligand Assay; RADIORECEPTOR ASSAY; REACTION KINETICS; RECEPTORS; SKIN; Temperature; TEMPERATURE DEPENDENCE; TIME DEPENDENCE; TISSUES; TRACER TECHNIQUES; Tumors; VERTEBRATES; Human; Membrane receptor; Carcinoma; Epidermal growth factor; Cell line; Radioimmunoassay; Digestive diseases; Tumor; Scatchard plot; Pancreas
• ISSN: 0885-3177; 1536-4828
• DOI: 10.1097/00006676-199005000-00006

The role of epidermal growth factor (EGF) in oncogenesis and progression of malignant tumors is a subject of vast interest. In this study, radioimmunoassay and radioreceptor assay of EGF were established. EGF contents in malignant and benign pancreatic tumors, in normal pancreas tissue, and in culture media of a human pancreatic carcinoma cell line were determined. EGF receptor binding studies were performed. It was shown that EGF contents in pancreatic carcinomas were significantly higher than those in normal pancreas or benign pancreatic tumors. EGF was also detected in the culture medium of a pancreatic carcinoma cell line. The binding of 125I-EGF to the pancreatic carcinoma cells was time and temperature dependent, reversible, competitive, and specific. Scatchard analysis showed that the dissociation constant of EGF receptor was 2.1 X 10(-9) M, number of binding sites was 1.3 X 10(5) cell. These results indicate that there is an over-expression of EGF/EGF receptors in pancreatic carcinomas, and that an autocrine regulatory mechanism may exist in the growth-promoting effect of EGF on tumor cells.