Effects of hydrophilic monomers on sorptive properties of divinylbenzene-based reversed phase sorbents

• Published in: Talanta (Oxford) Vol. 185; pp. 427 - 432
• Main Authors: Murakami, Hiroya, Aoyanagi, Takuya, Miki, Yuta, Tomita, Hiroki, Esaka, Yukihiro, Inoue, Yoshinori, Teshima, Norio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-08-2018
• Subjects: Hydrophilic lipophilic balance; Hydrophilic monomer; Methacrylate; Reversed phase sorbent; Hydrophilic monomer; Reversed phase sorbent; Hydrophilic lipophilic balance; Methacrylate
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2018.03.093

Solid phase extraction (SPE) has been extensively used as a pretreatment method. In SPE methods, commercially available reversed phase type sorbents, which consist of macroporus styrene-divinylbenzene or copolymers including divinylbenzene (DVB) and hydrophilic monomers, have been applied to a variety of samples. The later sorbents are called hydrophilic lipophilic balanced (HLB) type sorbents. Hydrophilic monomers in hydrophilic lipophilic balanced type sorbents contribute to the increase in retention of polar compounds, because hydrophilic monomers improve the wettability and increase the interaction with polar compounds as analytes. In this study, three different methacrylate monomers (ethylene glycol dimethacrylate (EGDMA), glycerol dimethacrylate (GDMA) and trimethylolpropane trimethacrylate (TMPTMA)), which are expected to improve the retention of polar compounds, were chosen, and DVB-based copolymetric sorbents including the three monomers were newly synthesized. Among them, the sorbents including GDMA or TMPTMA gave higher recoveries to polar compounds such as uridine and adenine than that including EGDMA. The optimization studies of hydrophilic lipophilic balance, inert diluent and the purity of DVB improved the sorptive abilities of the sorbents. The developed sorbents have higher recoveries for variety of polar compounds (cytosine, uracil, cytidine, uridine, 2′-deoxycytidine, 2′-deoxyguanosine, adenine, thymidine, adenosine and 2′-deoxyadenosine) than commercially available hydrophilic lipophilic balanced type sorbents, while the recoveries for theophylline were comparable between the proposed sorbents and the commercial sorbents.
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•We developed novel hydrophilic lipophilic balanced (HLB) type sorbents.•Divinylbenzene-based sorbents including different hydrophilic monomers were synthesized.•The HLB and inert diluent were optimized to improve the sorptive properties.•High recoveries for polar compounds can be achieved compared with commercial sorbents.