Selective distinction at equilibrium between the two α‐neurotoxin binding sites of Torpedo acetylcholine receptor by microtitration

The binding of the monoiodinated α‐neurotoxin I from Naja mossambica mossambica to the membrane‐bound acetylcholine receptor from Torpedo marmorata was investigated using a new picomolar‐sensitive microtitration assay. From equilibrium binding studies a non‐linear Scatchard plot demonstrated two pop...

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Bibliographic Details
Published in:	European journal of biochemistry Vol. 174; no. 3; pp. 537 - 542
Main Authors:	MARCHOT, Pascale, FRACHON, Paule, BOUGIS, Pierre E.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-06-1988 Blackwell
Subjects:	alpha -neurotoxin I Animal biology Animals Binding Sites Binding, Competitive Biological and medical sciences Bungarotoxins - metabolism Cell physiology Elapid Venoms - metabolism Electric Organ - metabolism electric organs Fundamental and applied biological sciences. Psychology Kinetics Life Sciences Mathematics Molecular and cellular biology Naja mossambica mossambica Neurotoxins - metabolism Neurotransmission Receptors, Cholinergic - metabolism Torpedo Torpedo marmorata Tubocurarine - metabolism Vertebrata Cholinergic receptor Pisces Neurotoxin Molecular interaction Electric organ Binding site
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