Effects of Flagellin on the Functions of Follicle-Associated Epithelium

• Published in: The Journal of infectious diseases Vol. 198; no. 6; pp. 907 - 910
• Main Authors: Chabot, Sophie M., Shawi, May, Eaves-Pyles, Tonyia, Neutra, Marian R.
• Format: Journal Article
• Language: English
• Published: Oxford The University of Chicago Press 15-09-2008; University of Chicago Press; Oxford University Press
• Subjects: Agonists; Animals; Antigens; Bacteria; Biological and medical sciences; Chemokines; Dendritic cells; Epithelial cells; Epithelial Cells - drug effects; Epithelial Cells - immunology; Epithelial Cells - physiology; Epithelium; Female; Flagellin - genetics; Flagellin - pharmacology; Fundamental and applied biological sciences. Psychology; Immunity, Mucosal; Infectious diseases; Matrix Metalloproteinases - metabolism; Medical sciences; Mice; Mice, Inbred BALB C; Microbiology; Peyers patches; Polymerase Chain Reaction; Recombinant Proteins - pharmacology; Salmonella - physiology; Salmonella dublin; T lymphocytes; Toll like receptors; Vaccination; Infection; Flagellin; Microbiology; Epithelium
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/591056

Bacterial flagellin activates innate immune responses by signaling through Toll-like receptor 5 and is a potential vaccine adjuvant. Mucosal lymphoid follicles, inductive sites for adaptive mucosal immune responses, are covered by a follicleassociated epithelium (FAE) specialized for the uptake of antigens. This study demonstrates that mucosal application of Salmonella dublin flagellin enhanced transepithelial transport of microparticles by the FAE of mouse Peyer's patches in vivo. Flagellin also induced rapid, matrix metalloproteinase—dependent migration of subepithelial dendritic cells (DCs) into the FAE, better positioning DCs for antigen capture. These innate responses to flagellin enhance FAE functions and may promote adaptive immune responses in the mucosa.