Recombinant Human Granulocyte Colony-Stimulating Factor Accelerates Hematopoietic Recovery After DLA-Identical Littermate Marrow Transplants in Dogs

• Published in: Blood Vol. 76; no. 3; pp. 636 - 640
• Main Authors: Schuening, Friedrich G., Storb, Rainer, Goehle, Sondra, Graham, Theodore C., Hackman, Robert, Mori, Motomi, Souza, Lawrence M., Appelbaum, Frederick R.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01-08-1990; The Americain Society of Hematology
• Subjects: Animals; Biological and medical sciences; Blood Cell Count - drug effects; Bone Marrow - drug effects; Bone Marrow - immunology; Bone Marrow Cells; Bone Marrow Transplantation - immunology; Bone Marrow Transplantation - pathology; Colony-Stimulating Factors - immunology; Colony-Stimulating Factors - pharmacology; Cross Reactions; Dogs; Female; Graft Rejection - drug effects; Graft Rejection - immunology; Graft vs Host Disease - epidemiology; Graft vs Host Disease - immunology; Graft vs Host Disease - prevention & control; Granulocyte Colony-Stimulating Factor; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - immunology; Histocompatibility Antigens - immunology; Histocompatibility Antigens Class I; Immunomodulators; Incidence; Male; Medical sciences; Pharmacology. Drug treatments; Recombinant Proteins - immunology; Recombinant Proteins - pharmacology; Fissipedia; Carnivora; Cytokine; Homograft; Hemopathy; Result; Vertebrata; Granulocyte colony stimulating factor; Experimental disease; Mammalia; Treatment; Hematopoiesis; Animal; Immunotherapy; Bone marrow; Graft; DLA-System; Dog
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V76.3.636.636

We studied whether treatment of dogs with recombinant human granulocyte colony-stimulating factor (rhG-CSF), after 920 cGy total body irradiation (TBI) and transplantation of 3.3 ± 1.0 × 108 bone marrow cells per kilogram from a DLA-identical littermate, accelerated hematopoietic recovery and influenced the incidence of subsequent marrow graft failure or graft-versus-host disease (GVHD). Ten animals were treated with 100 μg rhG-CSF/kg/d from days 1 through 10 after TBI. Results were compared with those of a historical control of 14 dogs not administered rhG-CSF. Neither group of dogs received GVHD prophylaxis. The median time to recovery of 1,000 neutrophils/mm3 was 8 days for dogs administered rhG-CSF compared with 14 days in controls (logrank test: P < .03). The median time to reach 100 monocytes/mm3 was 17 days in G-CSF-treated dogs compared with 49 days in controls [P < .002). The median time to attain 500 lymphocytes/mm3 was 15 days versus 31 days, respectively (P < .01). The median time to reach 20,000 platelets/mm3 was 26 versus 20 days (P = .68). Graft failure occurred in 1 of 10 G-CSF-treated dogs versus 2 of 14 controls (two-tailed Fisher's exact test: P = 1.00). GVHD was seen in 4 of 9 rhG-CSF-treated dogs compared with 1 of 12 controls (P = .12). Two G-CSF-treated dogs died of GVHD versus none of the controls (P = .17). No unusual toxicities were seen in dogs receiving rhG-CSF. In summary, rhG-CSF significantly accelerated recovery of neutrophils, monocytes, and lymphocytes after DLA-identical littermate marrow transplantation without altering platelet recovery. Graft failure was not seen more often than in controls, but there was a trend toward an increased incidence of GVHD.