Site-specific restriction endonuclease from the filamentous cyanobacterium Nostoc sp. MAC PCC 8009

We report here the presence of a type-II restriction endonuclease in the filamentous cyanobacterium Nostoc sp. MAC PCC 8009. This restriction enzyme, NSp MAC I, is the first reported isoschizomer of BglII and is very readily purified from non-specific deoxyribonuclease activity in the crude lysate b...

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Bibliographic Details
Published in:	FEBS letters Vol. 179; no. 1; pp. 129 - 132
Main Authors:	Lau, Reginald H., Visentin, Louis P., Martin, Stan M., Hofman, Jason D., Doolittle, W.Ford
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 1985 Elsevier
Subjects:	Analytical, structural and metabolic biochemistry Bgl II isoschizomer BglII isoschizomer Biological and medical sciences Cyanobacteria Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Nostoc Nsp MAC I Phosphocellulose column chromatography Nsp MAC I Phosphocellulose column chromatography Bgl II isoschizomer Cyanobacteria Site specificity Bacteria Purification Enzyme Restriction endonuclease
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Summary:	We report here the presence of a type-II restriction endonuclease in the filamentous cyanobacterium Nostoc sp. MAC PCC 8009. This restriction enzyme, NSp MAC I, is the first reported isoschizomer of BglII and is very readily purified from non-specific deoxyribonuclease activity in the crude lysate by one round of phosphocellulose column chromatography.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)80206-4