Molecular Cloning of the cDNA for Human Erythrocyte β-Spectrin

• Published in: Blood Vol. 72; no. 1; pp. 328 - 334
• Main Authors: Winkelmann, John C., Leto, Thomas L., Watkins, Paul C., Eddy, Roger, Shows, Thomas B., Linnenbach, Alban J., Sahr, Kenneth E., Kathuria, Navneet, Marchesi, Vincent T., Forget, Bernard G.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01-07-1988; The Americain Society of Hematology
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cell membranes. Ionic channels. Membrane pores; Cell structures and functions; Chromosome Mapping; Chromosomes, Human, Pair 14; Cloning, Molecular; DNA - isolation & purification; Fundamental and applied biological sciences. Psychology; Genes; Humans; Mice; Molecular and cellular biology; Molecular Sequence Data; Repetitive Sequences, Nucleic Acid; RNA, Messenger - isolation & purification; Spectrin - genetics; Spectrin - isolation & purification; Genetic mapping; Human; Spectrin; Complementary DNA; Gene; D14-Chromosome; Red blood cell; Cytoskeleton; Molecular cloning
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V72.1.328.328

Overlapping cDNA clones, totaling 3.3 kilobases (kb) in length, which encode over 50% of the human erythrocyte β-spectrin subunit, were isolated by antibody screening of a λ gt11 expression library constructed from human fetal liver mRNA. The amino acid sequence of the C-terminus of β-spectrin was derived. The size of β-spectrin mRNA in human erythroleukemia cells was found to be 7.5 kb. Erythrocyte β-spectrin is encoded by a gene located on human chromosome 14, as determined by cDNA hybridization to human X mouse somatic cell hybrids.