Triple aldose reductase/α-glucosidase/radical scavenging high-resolution profiling combined with high-performance liquid chromatography–high-resolution mass spectrometry–solid-phase extraction–nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude extract of Radix Scutellariae

•High-resolution recombinant human aldose reductase assay developed.•High-resolution bioactivity-profiling performed with Radix Scutellariae as proof of concept.•Triple aldose reductase/α-glucosidase/radical scavenging profiles obtained.•High-resolution profiles used for pinpointing antidiabetic con...

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 1408; pp. 125 - 132
Main Authors:	Tahtah, Yousof, Kongstad, Kenneth T., Wubshet, Sileshi G., Nyberg, Nils T., Jønsson, Louise H., Jäger, Anna K., Qinglei, Sun, Staerk, Dan
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 21-08-2015
Subjects:	Aldehyde Reductase - metabolism Aldose reductase alpha-Glucosidases - metabolism Chromatography Chromatography, High Pressure Liquid Free Radical Scavengers - analysis Glycoside Hydrolase Inhibitors - analysis High-resolution bioactivity profiling HPLC–HRMS–SPE–NMR Humans Hypoglycemic Agents - analysis Liquids Magnetic resonance Magnetic Resonance Spectroscopy - methods Plant Extracts - analysis Profiling Radical scavenging Radicals Radix Scutellariae Recombinant Proteins - metabolism Reductases Scavenging Scutellaria baicalensis - chemistry Solid Phase Extraction Spectroscopy α-Glucosidase HPLC–HRMS–SPE–NMR α-Glucosidase Aldose reductase Radix Scutellariae Radical scavenging High-resolution bioactivity profiling
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