Multiple insecticide resistance in Aedes aegypti (Diptera: Culicidae) from Boyeros municipality, Cuba and associated mechanisms

• Published in: Acta tropica Vol. 212; p. 105680
• Main Authors: Rodríguez, M.M., Ruiz, A., Piedra, L., Gutierrez, G., Rey, J., Cruz, M., Bisset, J.A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-12-2020
• Subjects: Aedes - drug effects; Aedes - genetics; Aedes aegypti; Animals; Cuba; Insecticide Resistance; Mechanisms of resistance; Mosquito Control; Pyrethrins - pharmacology; Sodium channel gene mutations; Sodium Channels - genetics; Temefos - pharmacology; Cuba; Sodium channel gene mutations; Aedes aegypti; Insecticide resistance; Mechanisms of resistance
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2020.105680

•Temephos resistance in larvae of Aedes aegypti was mainly associated with high frequency of α-esterase and GST activities and less associated with β- esterase and multi-function oxidase activities.•The presence of two mutations, V1016I and F1534C, in the sodium channel gene were detected for the first time in Ae. aegypti field populations from Cuba and were associated with a high level of resistance to deltamethrin.•The presence of both insecticide resistance mechanisms represents a limiting factor in the success of the control operations of Aedes aegypti.
In this study, insecticide resistance and the mechanisms responsible were characterized in Ae. aegypti of Boyeros municipality from Havana, Cuba. Boyeros represents a high epidemiological risk because it is located near the Havana International Airport, it is highly urbanized, and it has a large influx of people from endemic countries so that it qualifies as a sentinel site for surveillance. The larvae collected from five areas of this municipality showed resistance to temephos associated with metabolic enzymes. The adult mosquitoes displayed a deltamethrin resistance and less distinctly to other pyrethroids associated with a high frequency of sodium channel gene mutations (F1534C and V1016I), detected for the first time in a field population from Cuba. The presence in the field populations of two insecticide resistance mechanisms represents a limiting factor in the success of the control operations of this vector, so other strategies should be considered to preserve the effectiveness of the insecticides available in public health for vector control in Cuba.