Effects of combined microplastic and cadmium pollution on sorghum growth, Cd accumulation, and rhizosphere microbial functions

• Published in: Ecotoxicology and environmental safety Vol. 277; p. 116380
• Main Authors: Duan, Li-Yang, Zhang, Yu, Li, Yu-Ying, Li, Xiao-Qi, Liu, Yong-Qi, Li, B. Larry, Ding, Chuan-Yu, Ren, Xue-Min, Duan, Peng-Fei, Han, Hui, Chen, Zhao-Jin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-06-2024; Elsevier
• Subjects: Cadmium (Cd); Environmental risk; Metagenomics; Nitrogen and phosphorus cycling; Polystyrene (PS) and polyethylene (PE); Metagenomics; Cadmium (Cd); Environmental risk; Polystyrene (PS) and polyethylene (PE); Nitrogen and phosphorus cycling
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116380

The interaction between microplastics (MPs) and cadmium (Cd) poses a threat to agricultural soil environments, and their effects on plant growth and rhizosphere microbial community functions are not yet clear. In this study, energy sorghum was used as a test plant to investigate the effects of two types of MPs, polystyrene (PS) and polyethylene (PE), at different particle sizes (13 μm, 550 μm) and concentrations (0.1%, 1% w/w), and Cd, as well as their interactions, on the growth of sorghum in a soil-cultivation pot experiment. The results showed that the combined effects of MP and Cd pollution on the dry weight and Cd accumulation rate in sorghum varied depending on the type, concentration, and particle size of the MPs, with an overall trend of increasing stress from combined pollution with increasing Cd content and accumulation. High-throughput sequencing analysis revealed that combined MP and Cd pollution increased bacterial diversity, and the most significant increase was observed in the abundance-based coverage estimator (ACE), Shannon, and Sobs indices in the 13 μm 1% PS+Cd treatment group. Metagenomic analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways revealed that 19 groups of metabolic pathways, including microbial metabolism and methane metabolism, differed significantly under combined MP and Cd pollution. Hierarchical clustering results indicated that Cd treatment and combined MP and Cd treatment affected the abundances of sorghum rhizosphere soil nitrogen (N) and phosphorus (P) cycling genes and that the type of MP present was an important factor affecting N and P cycling genes. The results of this study provide a basis for exploring the toxic effects of combined MP and Cd pollution and for conducting soil environmental risk assessments.
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•Combined MP and Cd treatment tended to increase stress in sorghum plants.•Combined MP and Cd treatment increased Cd content and accumulation in sorghum.•Metagenomic analysis was used to determine the effects of combined MP and Cd treatment on rhizosphere microbial functions.•Combined MP and Cd treatment changed rhizosphere bacterial diversity and community composition.•Combined MP and Cd treatment altered microbial functions.