Parapseudoflavitalea muciniphila gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a human peritoneal tumour and reclassification of Pseudobacter ginsenosidimutans as Pseudoflavitalea ginsenosidimutans comb. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 6; pp. 3639 - 3646
• Main Authors: Lawson, Paul A, Patel, Nisha B, Mohammed, Ahmed, Moore, Edward R B, Lo, Alexander S, Sardi, Armando, Davis, Jeffrey M, Doyle, D Annie, Hui, Yvonne, Testerman, Traci
• Format: Journal Article
• Language: English
• Published: England Microbiology Society 01-06-2020
• Subjects: acid; bacteria; Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - isolation & purification; Baltimore; Base Composition; cancer; Chitinophagaceae; DNA, Bacterial - genetics; Fatty Acids - chemistry; Glycolipids - chemistry; Humans; Microbiology; Microbiology in the medical area; Mikrobiologi inom det medicinska området; Parapseudoflavitalea; Peritoneal Neoplasms - microbiology; Phosphatidylethanolamines - chemistry; Phylogeny; Pseudoflavitalea; Pseudomyxoma peritonei; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequences; Taxonomic Description; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Baltimore; cancer; Parapseudoflavitalea; Pseudoflavitalea; Pseudomyxoma peritonei; Chitinophagaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/IJSEM.0.004204

A Gram-stain-negative, microaerophilic, non-motile, rod-shaped bacterium strain designated PMP191F
, was isolated from a human peritoneal tumour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the organism formed a lineage within the family
that was distinct from members of the genus
(95.1-95.2 % sequence similarity) and
(94.4 % sequence similarity). The average nucleotide identity values between strain PMP191F
and
T16R-265
and
Gsoil 221
was 68.9 and 62.3% respectively. The only respiratory quinone of strain PMP191F
was MK-7 and the major fatty acids were iso-C
, iso-C
G and summed feature 3 (C
ω7
and/or C
ω6
). The polar lipids consisted of phosphatidylethanolamine and some unidentified amino and glycolipids. The G+C content of strain PMP191F
calculated from the genome sequence was 43.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, strain PMP191F
represents a novel species and genus for which the name
gen. nov., sp. nov. is proposed. The type strain is PMP191F
(=DSM 104999
=ATCC BAA-2857
= CCUG 72691
). The phylogenetic analyses also revealed that
shared over 98 % sequence similarly to members of the genus
. However, the average nucleotide identity value between
T16R-265
, the type species of the genus and
Gsoil 221
was 86.8 %. Therefore, we also propose that
be reclassified as
comb. nov.