CD11b/CD18-coated microspheres attach to E-selectin under flow

• Published in: Journal of leukocyte biology Vol. 67; no. 2; pp. 196 - 205
• Main Authors: Crutchfield, Karen L., Patil, Vivek R. Shinde, Campbell, Craig J., Parkos, Charles A., Allport, Jennifer R., Goetz, Douglas J.
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01-02-2000
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; CD11b antigen; CD18 antigen; CD18 Antigens - immunology; CD18 Antigens - metabolism; CD62E; Cell Adhesion - drug effects; Cells, Cultured; CHO Cells - cytology; CHO Cells - metabolism; Cricetinae; Cricetulus; E-selectin; E-Selectin - genetics; E-Selectin - metabolism; endothelium; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Fibrinogen - metabolism; Gangliosides - metabolism; Glycosylation; Humans; inflammation; integrins; leukocyte; Macrophage-1 Antigen - immunology; Macrophage-1 Antigen - metabolism; Mac‐1; Microspheres; Neuraminidase - pharmacology; Protein Processing, Post-Translational; Recombinant Fusion Proteins - metabolism; Rheology; Transfection; Umbilical Veins
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.67.2.196

Neutrophils can attach to E‐selectin under flow. Proposed ligands for E‐selectin carry SLex‐type glycans. The leukocyte β2 integrins are glycosylated with SLex. Thus, we speculated that β2 integrins could support attachment to E‐selectin. To test this hypothesis, we coated 10–diameter microspheres with purified CD11b/CD18 (αMβ2) and investigated the adhesion of the resulting αMβ2 microspheres to E‐selectin. Under in vitro flow conditions, the αMβ2 microspheres attached to Chinese hamster ovary cells expressing E‐selectin (CHO‐E) and 4‐h interleukin‐1 β‐activated human umbilical vein endothelial cells (HUVEC). At a shear stress of 1.8 dynes/cm2, the attachment events were eliminated by pretreatment of the cellular monolayers with a mAb to E‐selectin. microspheres did not attach to untransfected CHO cells or unactivated HUVEC at 1.8 dynes/cm2. Taken together, the results strongly suggest that the CD11b/CD18‐E‐selectin bond has sufficient biophysical properties to mediate attachment of neutro‐phil‐sized particles to E‐selectin under flow. J. Leukoc. Biol. 67: 196–205; 2000.