Human islet isolation outcomes from pancreata preserved with histidine-tryptophan ketoglutarate versus university of wisconsin solution

• Published in: Transplantation Vol. 82; no. 7; pp. 983 - 985
• Main Authors: SALEHI, Payam, HANSEN, Michael A, OBERHOLZER, Jose, AVILA, Jose G, BARBARO, Barbara, GANGEMI, Antonio, ROMAGNOLI, Travis, YONG WANG, MEIRIGENG QI, MURDOCK, Philip, BENEDETTI, Enrico
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott 15-10-2006
• Subjects: Adenosine; Adult; Allopurinol; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glucose; Glutathione; Humans; Insulin; Islets of Langerhans - cytology; Male; Mannitol; Medical sciences; Middle Aged; Organ Preservation Solutions; Pancreas - cytology; Potassium Chloride; Procaine; Raffinose; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue Donors - statistics & numerical data; Tissue, organ and graft immunology; Human; Organ preservation; Histidine; Prognosis; Aminoacid; Langerhans islet; Tryptophan; Evolution; Graft; Isolation; Comparative study; Endocrine pancreas
• ISSN: 0041-1337; 1534-6080
• DOI: 10.1097/01.tp.0000232310.49237.06

This study was designed to compare Histadine-Tryptophan-Ketogluterate (HTK) with University of Wisconsin (UW) solution. Pancreata from extended criteria donors were flushed and transported with HTK (n=41) or UW (n=45). Isolation outcomes were determined by islet yields, viability and in vitro and in vivo function. Final yields were similar between two groups (HTK: 383,085 vs. UW: 328,514 EIN, P=0.14). In the HTK group, 63.4% (26/41) of isolations resulted in a yield of over 300,000, and in the UW group this was achieved in 46.7% (21/45; P=0.12). Viability results were similar (HTK: 82.9 vs. UW: 82.7%, P=0.93). Stimulation index in the HTK and UW groups were comparable (5.28 vs. 4.91, P=0.62). Ten out of 41 islet preparations in HTK and 4 of 45 in UW group were suitable for clinical transplantation (P=0.05). Our study shows HTK is equivalent to UW solution in the preservation of pancreata for islet isolation.