Induction of shikimic acid pathway enzymes by light in suspension cultured cells of parsley (Petroselinum crispum)

• Published in: Plant physiology (Bethesda) Vol. 94; no. 2; pp. 507 - 510
• Main Authors: McCue, K.F. (Michigan State University, East Lansing, MI), Conn, E.E
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01-10-1990
• Subjects: ACIDE ORGANIQUE; ACIDOS ORGANICOS; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; Biosynthesis; Cell culture techniques; CULTIVO DE CELULAS; CULTURE DE CELLULE; Cultured cells; Cyclic amino acids; Enzymes; FENILALANINA; FLAVONOIDE; FLAVONOIDES; Flavonoids; Fundamental and applied biological sciences. Psychology; Gene induction; LIGASAS; LIGASE; LUMIERE; LUZ; Metabolism; Nitrogen metabolism; Parsley; PETROSELINUM CRISPUM; PHENYLALANINE; Plant physiology and development; Plants; Protein isoforms; VIA BIOQUIMICA DEL METABOLISMO; VOIE BIOCHIMIQUE DU METABOLISME; Cell culture; Light effect; Phenylalanine; Enzyme; Isozyme; Petroselinum crispum; Biosynthesis; Shikimic acid; Enzymatic activity; Chorismate mutase; Dicotyledones; Angiospermae; Flavoring crop; Isolation; Spermatophyta; Umbelliferae
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.94.2.507

Light treatment of suspension cultured cells of parsley (Petroselinum crispum) was shown to increase the activity of the shikimic acid pathway enzyme, 3-deoxy-D-arabino-heptulosonic acid-7-phosphate (DAHP) synthase (EC 4.1.2.15). DAHP synthase activity was assayed for two isoforms, DS-Mn and DS-Co (RJ Ganson, TA d'Amato, RA Jensen [1986] Plant Physiol 82: 203-210). Light increased the enzymatic activity of the plastidic isoform DS-Mn as much as 2-fold, averaging 1.6-fold with 95% confidence. The cytosolic isoform DS-Co was unaffected. Cycloheximide and actinomycin D, translational and transcriptional inhibitors, respectively, both reversed induction of DS-Mn by light suggesting transcriptional regulation of the gene. Chorismate mutase activity was assayed for the two isoforms CM I and CM II (BK Singh, JA Connelly, EE Conn [1985] Arch Biochem Biophys 243: 374-384). Treatment by light did not significantly affect either chorismate mutase isoform. The ratio of the two chorismate mutase isoforms changed during the growth cycle, with an increase in the ratio of plastidic to cytosolic isoforms occurring towards the end of logarithmic growth