Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ

[Display omitted] ► A real-time PCR method for the detection in situ of Anisakis pegreffii was developed. ► The ITS-2 of rDNA of A. pegreffii was used as molecular marker. ► The assay was capable of detecting 1/3 L3 in 30mg of fish tissue. To facilitate improved diagnosis and detection of the third...

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Bibliographic Details
Published in:	Experimental parasitology Vol. 127; no. 2; pp. 587 - 592
Main Authors:	Fang, Wenzhen, Liu, Fan, Zhang, Shaolei, Lin, Junhua, Xu, Shisan, Luo, Damin
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-02-2011 Elsevier
Subjects:	Animals Anisakiasis - diagnosis Anisakiasis - parasitology Anisakiasis - veterinary Anisakis Anisakis - genetics Anisakis - isolation & purification Anisakis pegreffii Biological and medical sciences DNA Primers DNA Probes DNA, Ribosomal DNA, Ribosomal Spacer - genetics Fish Diseases - diagnosis Fish Diseases - parasitology Fishes Fluorescent Dyes Food-borne parasitic zoonosis Fundamental and applied biological sciences. Psychology In situ Invertebrates Life cycle. Host-agent relationship. Pathogenesis Marine Nemathelminthia. Plathelmintha Nematode Polymerase Chain Reaction - methods Protozoa Reproducibility of Results Second internal transcribed spacer (ITS-2) Sensitivity and Specificity TaqMan real-time PCR ISEW, Pacific, Taiwan Strait ISEW, China, People's Rep., Minnan-Taiwan Bank Anisakis pegreffii Nematode Second internal transcribed spacer (ITS-2) TaqMan real-time PCR Food-borne parasitic zoonosis In situ Fluorescence Parasite Zoonosis Infection Polymerase chain reaction Helmintha Nemathelminthia Invertebrata Detection Nematoda
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