Increased 5-HT2C receptor binding in the brain stem and cerebral cortex during liver regeneration and hepatic neoplasia in rats

• Published in: Journal of the neurological sciences Vol. 254; no. 1; pp. 3 - 8
• Main Authors: Pyroja, Sulaiman, Joseph, Binoy, Paulose, Chiramadathikudiyil S
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Science 15-03-2007
• Subjects: Animals; Binding, Competitive; Biological and medical sciences; Brain - metabolism; Brain Stem - metabolism; Carcinogens; Cell Proliferation; Cerebral Cortex - metabolism; Diethylnitrosamine; Hepatocytes - metabolism; Injuries of the nervous system and the skull. Diseases due to physical agents; Liver - innervation; Liver - metabolism; Liver Neoplasms, Experimental - chemically induced; Liver Neoplasms, Experimental - metabolism; Liver Regeneration; Male; Medical sciences; Neurology; Norepinephrine - metabolism; Rats; Rats, Wistar; Receptor, Serotonin, 5-HT2C - metabolism; Serotonin - metabolism; Sympathetic Fibers, Postganglionic - metabolism; Synaptic Membranes - metabolism; Traumas. Diseases due to physical agents; Up-Regulation; Vascular diseases and vascular malformations of the nervous system; Hepatic neoplasia; Cerebral cortex; 5-HT 2C; Partial hepatectomy; Brain stem; Liver regeneration; Nervous system diseases; Rat; Digestive system; Liver; Rodentia; Central nervous system; Encephalon; Regeneration; Vertebrata; Mammalia; Animal; 5-HT2C; 5-HT2C serotonin receptor
• ISSN: 0022-510X; 1878-5883
• DOI: 10.1016/j.jns.2006.12.003

Abstract In the present study, serotonin 2C (5-HT2C ) receptor binding parameters in the brainstem and cerebral cortex were investigated during liver generation after partial hepatectomy (PH) and N -nitrosodiethylamine (NDEA) induced hepatic neoplasia in male Wistar rats. The serotonin content increased significantly ( p < 0.01) in the cerebral cortex after PH and in NDEA induced hepatic neoplasia. Brain stem serotonin content increased significantly ( p < 0.05) after PH and ( p < 0.001) in NDEA induced hepatic neoplasia. The number and affinity of the 5-HT2C receptors in the crude synaptic membrane preparations of the brain stem showed a significant ( p < 0.001) increase after PH and in NDEA induced hepatic neoplasia. The number and affinity of 5-HT2C receptors increased significantly ( p < 0.001) in NDEA induced hepatic neoplasia in the crude synaptic membrane preparations of the cerebral cortex. There was a significant ( p < 0.01) increase in plasma norepinephrine in PH and ( p < 0.001) in NDEA induced hepatic neoplasia, indicating sympathetic stimulation. Thus, our results suggest that during active hepatocyte proliferation 5-HT2C receptor in the brain stem and cerebral cortex are up-regulated which in turn induce hepatocyte proliferation mediated through sympathetic stimulation.