Transmission of a New Polerovirus Infecting Pepper by the Whitefly Bemisia tabaci

• Published in: Journal of virology Vol. 93; no. 15
• Main Authors: Ghosh, Saptarshi, Kanakala, Surapathrudu, Lebedev, Galina, Kontsedalov, Svetlana, Silverman, David, Alon, Tamar, Mor, Neta, Sela, Noa, Luria, Neta, Dombrovsky, Aviv, Mawassi, Munir, Haviv, Sabrina, Czosnek, Henryk, Ghanim, Murad
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01-08-2019
• Subjects: Animals; Capsicum - parasitology; Capsicum - virology; Cellular Response to Infection; Hemiptera - virology; Insect Vectors - virology; Israel; Luteoviridae - classification; Luteoviridae - genetics; Luteoviridae - isolation & purification; Phylogeny; Plant Diseases - virology; Sequence Homology; Israel; transmission; polerovirus; Bemisia tabaci; circulative; aphid
• DOI: 10.1128/JVI.00488-19
• EISSN: 1098-5514
• ISSN: 0022-538X; 1098-5514

Many animal and plant viruses depend on arthropods for their transmission. Virus-vector interactions are highly specific, and only one vector or one of a group of vectors from the same family is able to transmit a given virus. Poleroviruses (
) are phloem-restricted RNA plant viruses that are exclusively transmitted by aphids. Multiple aphid-transmitted polerovirus species commonly infect pepper, causing vein yellowing, leaf rolling, and fruit discoloration. Despite low aphid populations, a recent outbreak with such severe symptoms in many bell pepper farms in Israel led to reinvestigation of the disease and its insect vector. Here we report that this outbreak was caused by a new whitefly (
)-transmitted polerovirus, which we named Pepper whitefly-borne vein yellows virus (PeWBVYV). PeWBVYV is highly (>95%) homologous to
(PeVYV) from Israel and Greece on its 5' end half, while it is homologous to
(AeYV) on its 3' half. Koch's postulates were proven by constructing a PeWBVYV infectious clone causing the pepper disease, which was in turn transmitted to test pepper plants by
but not by aphids. PeWBVYV represents the first report of a whitefly-transmitted polerovirus.
The high specificity of virus-vector interactions limits the possibility of a given virus changing vectors. Our report describes a new virus from a family of viruses strictly transmitted by aphids which is now transmitted by whiteflies (
) and not by aphids. This report presents the first description of polerovirus transmission by whiteflies. Whiteflies are highly resistant to insecticides and disperse over long distances, carrying virus inoculum. Thus, the report of such unusual polerovirus transmission by a supervector has extensive implications for the epidemiology of the virus disease, with ramifications concerning the international trade of agricultural commodities.