Parasutterella excrementihominis gen. nov., sp. nov., a member of the family Alcaligenaceae isolated from human faeces

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 7; pp. 1793 - 1797
• Main Authors: Nagai, Fumiko, Morotomi, Masami, Sakon, Hiroshi, Tanaka, Ryuichiro
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-07-2009; Society for General Microbiology
• Subjects: Alcaligenaceae - classification; Alcaligenaceae - genetics; Alcaligenaceae - isolation & purification; Alcaligenaceae - physiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; DNA, Bacterial - analysis; Fatty Acids - analysis; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genotype; Humans; Japan; Microbiology; Molecular Sequence Data; Phenotype; Phylogeny; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species Specificity; Sutterella; Systematics; Japan; Human; Bacteria; New genus; New species; Feces; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.002519-0

Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 186-8650 Tokyo, Japan
Correspondence Fumiko Nagai fumiko-nagai{at}yakult.co.jp
A novel, strictly anaerobic, non-spore-forming, Gram-negative coccobacillus (strain YIT 11859 T ) was isolated from human faeces. Biochemically, this strain was largely unreactive and was asaccharolytic. Growth of strain YIT 11859 T in peptone-yeast extract broth produced no visible turbidity, and a trace amount of propionate was detected as an end product of metabolism. 16S rRNA gene sequence analysis showed that strain YIT 11859 T was related most closely to the type strains of Sutterella species, with 90.8–88.0 % sequence similarity. Phylogenetic analysis of these and other related sequences confirmed that strain YIT 11859 T was phylogenetically most closely associated with Sutterella species, but formed a separate cluster, indicating that strain YIT 11859 T represents a novel member of the family Alcaligenaceae . Fatty acid analysis demonstrated the presence of a high concentration of C 18 : 1 9 c (75 % of the total). The main respiratory quinones were menaquinone (MK–6) and methylated menaquinone (MMK-6). The G+C content of the DNA was 49.8 mol%. These results suggest that strain YIT 11859 T represents a novel species of a new genus, for which the name Parasutterella excrementihominis gen. nov., sp. nov. is proposed. The type strain of Parasutterella excrementihominis is YIT 11859 T (=DSM 21040 T =JCM 15078 T ).
Abbreviations: FAME, fatty acid methyl ester; ML, maximum likelihood; MP, maximum parsimony
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIT 11859 T is AB370250 .
Maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences and a table giving the cellular fatty acid compositions of strain YIT 11859 T and the type strains of related type species in the family Alcaligenaceae are available as supplementary material with the online version of this paper.