Human embryonic stem cells as a model system for studying the effects of smoke exposure on the embryo
Human embryonic stem cells (hESCs) share many characteristics including pluripotency with cells of the early embryo and so are potentially useful tools for studying the harmful effects of xenobiotics during early development. Here, we used hESCs as a model system to test the effects of nicotine on t...
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| Published in: | Reproductive toxicology (Elmsford, N.Y.) Vol. 26; no. 2; pp. 86 - 93 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
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01-10-2008
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| Abstract | Human embryonic stem cells (hESCs) share many characteristics including pluripotency with cells of the early embryo and so are potentially useful tools for studying the harmful effects of xenobiotics during early development. Here, we used hESCs as a model system to test the effects of nicotine on the pluripotent population of cells that forms the whole body. Specifically, we exposed hESCs (H7 and H9) to various concentrations of nicotine ranging from 0.1 to 6
μM. We evaluated the effects in terms of cell adhesion, integrin expression, hESC colony morphology, markers of pluripotency and survival. The results revealed a significant negative impact of nicotine in the dose range between 1.8 and 3.7
μM on all the endpoints analyzed. The observed effects were reversed by the addition of the nicotine antagonist
d-tubocurarine, suggesting that the effects are receptor mediated. Together these results offer new explanations in terms of embryo toxicity for the large negative impact of cigarette smoke exposure on a woman's reproductive capacity. |
|---|---|
| AbstractList | Human embryonic stem cells (hESCs) share many characteristics including pluripotency with cells of the early embryo and so are potentially useful tools for studying the harmful effects of xenobiotics during early development. Here, we used hESCs as a model system to test the effects of nicotine on the pluripotent population of cells that forms the whole body. Specifically, we exposed hESCs (H7 and H9) to various concentrations of nicotine ranging from 0.1 to 6kM. We evaluated the effects in terms of cell adhesion, integrin expression, hESC colony morphology, markers of pluripotency and survival. The results revealed a significant negative impact of nicotine in the dose range between 1.8 and 3.7kM on all the endpoints analyzed. The observed effects were reversed by the addition of the nicotine antagonist d-tubocurarine, suggesting that the effects are receptor mediated. Together these results offer new explanations in terms of embryo toxicity for the large negative impact of cigarette smoke exposure on a woman's reproductive capacity. Human embryonic stem cells (hESCs) share many characteristics including pluripotency with cells of the early embryo and so are potentially useful tools for studying the harmful effects of xenobiotics during early development. Here, we used hESCs as a model system to test the effects of nicotine on the pluripotent population of cells that forms the whole body. Specifically, we exposed hESCs (H7 and H9) to various concentrations of nicotine ranging from 0.1 to 6microM. We evaluated the effects in terms of cell adhesion, integrin expression, hESC colony morphology, markers of pluripotency and survival. The results revealed a significant negative impact of nicotine in the dose range between 1.8 and 3.7microM on all the endpoints analyzed. The observed effects were reversed by the addition of the nicotine antagonist d-tubocurarine, suggesting that the effects are receptor mediated. Together these results offer new explanations in terms of embryo toxicity for the large negative impact of cigarette smoke exposure on a woman's reproductive capacity. Human embryonic stem cells (hESCs) share many characteristics including pluripotency with cells of the early embryo and so are potentially useful tools for studying the harmful effects of xenobiotics during early development. Here, we used hESCs as a model system to test the effects of nicotine on the pluripotent population of cells that forms the whole body. Specifically, we exposed hESCs (H7 and H9) to various concentrations of nicotine ranging from 0.1 to 6 μM. We evaluated the effects in terms of cell adhesion, integrin expression, hESC colony morphology, markers of pluripotency and survival. The results revealed a significant negative impact of nicotine in the dose range between 1.8 and 3.7 μM on all the endpoints analyzed. The observed effects were reversed by the addition of the nicotine antagonist d-tubocurarine, suggesting that the effects are receptor mediated. Together these results offer new explanations in terms of embryo toxicity for the large negative impact of cigarette smoke exposure on a woman's reproductive capacity. |
| Author | LaRocque, Nicholas McMaster, Michael Fisher, Susan Genbacev, Olga Zdravkovic, Tamara |
| Author_xml | – sequence: 1 givenname: Tamara surname: Zdravkovic fullname: Zdravkovic, Tamara organization: Department of Cell and Tissue Biology, University of California, San Francisco, CA, USA – sequence: 2 givenname: Olga surname: Genbacev fullname: Genbacev, Olga organization: Department of Cell and Tissue Biology, University of California, San Francisco, CA, USA – sequence: 3 givenname: Nicholas surname: LaRocque fullname: LaRocque, Nicholas organization: Department of Cell and Tissue Biology, University of California, San Francisco, CA, USA – sequence: 4 givenname: Michael surname: McMaster fullname: McMaster, Michael organization: Department of Cell and Tissue Biology, University of California, San Francisco, CA, USA – sequence: 5 givenname: Susan surname: Fisher fullname: Fisher, Susan email: sfisher@cgl.ucsf.edu organization: Department of Cell and Tissue Biology, University of California, San Francisco, CA, USA |
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| Keywords | Human embryonic stem cells Integrin Adhesion OCT-3/4 Nicotine Maternal smoking Human Embryo Stem cell Cell adhesion molecule Transcription factor Oct4 Alkaloid Mother Embryonic cell Passive smoking Models |
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| SubjectTerms | Adhesion Apoptosis - drug effects Biological and medical sciences Biomarkers - metabolism Cell Adhesion - drug effects Cell Differentiation - drug effects Cell Line Dose-Response Relationship, Drug Embryology: invertebrates and vertebrates. Teratology Embryonic Development - drug effects Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism Embryonic Stem Cells - pathology Fundamental and applied biological sciences. Psychology Human embryonic stem cells Humans Immunohistochemistry In Situ Nick-End Labeling Integrin Maternal smoking Medical sciences Models, Biological Nicotine Nicotine - toxicity OCT-3/4 Pluripotent Stem Cells - cytology Pluripotent Stem Cells - drug effects Pluripotent Stem Cells - metabolism Pluripotent Stem Cells - pathology Smoking - adverse effects Teratology. Teratogens Tobacco, tobacco smoking Toxicology |
| Title | Human embryonic stem cells as a model system for studying the effects of smoke exposure on the embryo |
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