A single tube, quantitative real-time RT-PCR assay that detects four potato viruses simultaneously

A high throughput, real-time multiplex, single tube RT-PCR assay was developed for simultaneous detection of Potato leafroll virus (PLRV), Potato virus X (PVX) and Potato virus S (PVS) in potato leaves and tubers, and Tomato spotted wilt virus (TSWV) in potato tubers and tomato leaves. The test uses...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 161; no. 2; pp. 289 - 296
Main Authors:	Mortimer-Jones, Sheila M., Jones, Michael G.K., Jones, Roger A.C., Thomson, Gordon, Dwyer, Geoffrey I.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier B.V 01-11-2009 Elsevier
Subjects:	Biological and medical sciences Carlavirus - genetics Carlavirus - isolation & purification DNA Probes Fundamental and applied biological sciences. Psychology Luteoviridae - genetics Luteoviridae - isolation & purification Lycopersicon esculentum Lycopersicon esculentum - virology Microbiology Multiplex real-time RT-PCR Plant Leaves - virology Plant Roots - virology Plant Viruses - genetics Plant Viruses - isolation & purification Potato leafroll virus Potato leafroll virus (PLRV) Potato tubers Potato virus S Potato virus S (PVS) Potato virus X Potato virus X (PVX) Potexvirus - genetics Potexvirus - isolation & purification Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - genetics RNA, Viral - isolation & purification Sensitivity and Specificity Solanum tuberosum Solanum tuberosum - virology Techniques used in virology Tomato spotted wilt virus Tomato spotted wilt virus (TSWV) Tospovirus - genetics Tospovirus - isolation & purification Virology Multiplex real-time RT-PCR Potato leafroll virus (PLRV) Potato tubers Potato virus X (PVX) Potato virus S (PVS) Tomato spotted wilt virus (TSWV) Multiplex real-time RT―PCR Plant pathogen Microbiology Tomato spotted wilt virus Bunyaviridae Flexiviridae Dicotyledones Angiospermae Luteoviridae Solanaceae Reverse transcription polymerase chain reaction Quantitative analysis Multiplex polymerase chain reaction Solanum tuberosum Potexvirus Tospovirus Potato leafroll virus Method Real time Polerovirus Carlavirus Virus Potato virus X Spermatophyta Tuber plant Detection Potato virus S
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