The metal binding site of the major house dust mite allergen Der p 1

Enzyme kinetic measurements on Der p 1 are usually performed in the presence of EDTA to prevent cation-facilitated inactivation of the active site cysteine residue, and therefore the metal binding site is unlikely to be occupied under these conditions.

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Bibliographic Details
Published in:	Journal of allergy and clinical immunology Vol. 118; no. 4; p. 971
Main Authors:	Meno, Kåre, Thorsted, Peter B., Ipsen, Henrik, Kristensen, Ole, Larsen, Jørgen N., Spangfort, Michael D., Gajhede, Michael, Lund, Kaare
Format:	Journal Article
Language:	English
Published:	United States Mosby, Inc 01-10-2006 Elsevier Limited
Subjects:	Animals Antigens, Dermatophagoides - chemistry Antigens, Dermatophagoides - metabolism Arthropod Proteins Binding Sites Crystal structure Crystallization Cysteine Endopeptidases Enzyme kinetics Magnesium Metals - metabolism
Online Access:	Get full text
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Description
Summary:	Enzyme kinetic measurements on Der p 1 are usually performed in the presence of EDTA to prevent cation-facilitated inactivation of the active site cysteine residue, and therefore the metal binding site is unlikely to be occupied under these conditions.
Bibliography:	SourceType-Other Sources-1 content type line 63 ObjectType-Correspondence-1 ObjectType-Commentary-2
ISSN:	0091-6749 1097-6825
DOI:	10.1016/j.jaci.2006.06.038