The usage of micellar extraction for analysis of fluvastatin in water and wastewater samples

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 106; pp. 129 - 135
• Main Authors: Hryniewicka, Marta, Starczewska, Barbara
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15-03-2015
• Subjects: Calibration; Chromatography, High Pressure Liquid - methods; Fatty Acids, Monounsaturated - analysis; Fluvastatin; Hydrogen-Ion Concentration; Indoles - analysis; Limit of Detection; Micellar extraction; Micelles; Rivers; Sodium dodecylsulfate; Spectrophotometry, Ultraviolet - methods; Surface-Active Agents - chemistry; Tetra-n-butyloammonium bromide; Triton X114; Waste Water - analysis; Water Pollutants, Chemical - analysis; Triton X114; Fluvastatin; Sodium dodecylsulfate; Tetra-n-butyloammonium bromide; Micellar extraction
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2014.11.030

•Fluvastatin has created micelle with anionic surfactant sodium dodecylsulfate.•Mixture of triton X114 and tetrabutyloammonium bromide is proper for isolation of FLU.•The addition of a suitable salt as an electrolyte (NaCl and Na2SO4) is required.•Concentrations of surfactants, pH of sample, centrifugation time were investigated.
This work illustrates the development of new procedures for the isolation and preconcentration of fluvastatin (FLU) from aqueous solutions. Micellar extraction (ME) combined with high performance liquid chromatography (HPLC-UV) has been successfully applied for this purpose. It was found that the analyte created micelle with anionic sodium dodecylsulfate (SDS) and/or with the binary mixture of surfactants nonionic triton X114 (TX114) and cationic tetra-n-butyloammonium bromide (TBAB).
The optimal analytical conditions for the proposed extraction procedures (solution pH, concentration of surfactants, centrifugation time and electrolyte type) were ascertained. The calibration curves were recorded. The linearity ranges for FLU, isolated by SDS and the mixture of TX114/TBAB, were 0.21–28.79μgmL−1 and 0.21–16.45μgmL−1 with limit of detection (LOD) 0.19μgmL−1 and 0.14μgmL−1, respectively. The recoveries afforded by the proposed methods were high, approximately 97%. These preconcentration procedures were applied for the isolation of the statin from water and wastewater samples taken from the local rivers and wastewater treatment plants.