Optimization of an antibiotic residue screening test, based on inhibition of Bacillus subtilis BGA, with experimental design

• Published in: Food additives and contaminants Vol. 15; no. 5; p. 528
• Main Authors: Koenen-Dierick, K, De Beer, J O
• Format: Journal Article
• Language: English
• Published: England 01-07-1998
• Subjects: Animals; Anti-Bacterial Agents - analysis; Bacillus subtilis; Biological Assay; Colony Count, Microbial; Drug Residues; Food Contamination - analysis; Meat - analysis; Oxytetracycline - analysis; Streptomycin - analysis; Sulfamethazine - analysis; Tylosin - analysis; Veterinary Drugs - analysis
• ISSN: 0265-203X
• DOI: 10.1080/02652039809374677

Experimental design was applied to an agar-diffusion inhibition test for the screening of residues of veterinary antimicrobial drugs in slaughter animals. The effects and interactions of four independent parameters were studied: the dextrose content of the culture medium, the trimethoprim concentration in the culture medium, the thickness of the agar-layer and the preincubation time. The effects on the inhibition zones of sulphadimidine, oxytetracycline, streptomycin and tylosin, substances of four different antibiotic groups were measured. An optimized multi-residue test was established with the parameter values that yielded the best sensitivity for the four substances.