Investigation of the Multixenobiotic Resistance Mechanism in the Freshwater Fishes Western Mosquitofish, Gambusia affinis, and Bluegill Sunfish, Lepomis macrochirus

• Published in: Bulletin of environmental contamination and toxicology Vol. 83; no. 5; pp. 640 - 643
• Main Authors: Damaré, C. L, Kaddoumi, A. K, Baer, K. N
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01-11-2009; Springer-Verlag; Springer Nature B.V
• Subjects: Animals; Aquatic Pollution; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Biomarkers; Biomarkers - metabolism; Blotting, Western; Contaminants; Contamination; Culicidae; Detoxification; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Fish; Fishes; Fresh Water; Freshwater; Freshwater fish; Gambusia affinis; Gene expression; Glycoproteins; Lepomis macrochirus; Pisces; Pollution; Rhodamine 123 - pharmacology; Soil Science & Conservation; Species Specificity; Teleostei; Verapamil - toxicity; Waste Water Technology; Water Management; Water Pollutants, Chemical - toxicity; Water Pollution Control; Xenobiotics - toxicity; Bluegill sunfish; MXR; Mosquitofish; Glycoprotein
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-009-9846-x

The purpose of the study was to evaluate multixenobiotic resistance mechanism expression as a biomarker for contaminant exposure in freshwater fishes. Exposure to a known mammalian inhibitor (verapamil, 10 μM) and inducer (rhodamine 123, 3 μM) on the transport protein P-glycoprotein (Pgp) was investigated in the liver of the western mosquitofish and bluegill sunfish. No differences in the activity or expression of Pgp were measured in either species using a fluorometric accumulation assay and western blot analyses. The preliminary results from this study indicate that this detoxification mechanism may not be a sensitive indicator of contaminant exposure in certain teleost species.