PCR Screening of Enterococcus faecalis from Mixture of Lactic Acid Bacteria Using Bile Salt Hydrolase Gene as a Selective Marker

• Published in: Indian journal of microbiology Vol. 60; no. 2; pp. 251 - 253
• Main Authors: Yoon, Hye Min, Song, Su Jin, Ko, Ji Su, Yoon, Hye Kyoung, Kim, Su Jeong, Kim, So Jin, Kim, Ji Woon, Yoo, Min
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-06-2020; Springer Nature B.V
• Subjects: Bacteria; Bile; Biomedical and Life Sciences; Contamination; Enterococcus faecalis; Fermented food; Food contamination; Food poisoning; Freshness; Hydrolase; Kimchi; Lactic acid; Lactic acid bacteria; Life Sciences; Medical Microbiology; Microbiology; Screening; Short Communications; Food quarantine; PCR primer; Bile salt hydrolase gene; E. faecalis
• ISSN: 0046-8991; 0973-7715
• DOI: 10.1007/s12088-019-00851-9

It is very important to rapidly detect the contamination of
Enterococcus faecalis
in fermented foods such as Korean Kimchi to maintain its freshness since Kimchi is exported to all over the world. However, gene sequence of
E. faecalis
is very similar among various
Lactobacillus
. So, there have been difficulties in its screening. We have designed primers based on Bile salt hydrolase gene of
E. faecalis
and applied them to PCR test. PCR band was identified only from
E. faecalis
and only from the mixture contaminated with
E. faecalis.
It means that the primers we designed are highly specific for distinguishing contamination of
E. faecalis
. It will be possible to precisely screen within 1 h, which will greatly contribute to the prevention of food poisoning and quick quarantine.