Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticles

Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticles

A novel amplification route for DNA detection based on the deposition of gold on a 10 nm Au-colloid/avidin conjugate label acting as a 'seeding' catalyst, is described. Microgravimetric quartz-crystal-microbalance measurements are employed to transduce the catalyzed deposition of gold on t...

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Bibliographic Details
Published in:Analyst (London) Vol. 126; no. 9; p. 1502
Main Authors: Weizmann, Y, Patolsky, F, Willner, I
Format: Journal Article
Language:English
Published: England 01-09-2001
Subjects:
Base Pair Mismatch
DNA - analysis
DNA - genetics
Gold
Humans
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Description
Summary:A novel amplification route for DNA detection based on the deposition of gold on a 10 nm Au-colloid/avidin conjugate label acting as a 'seeding' catalyst, is described. Microgravimetric quartz-crystal-microbalance measurements are employed to transduce the catalyzed deposition of gold on the piezoelectric crystals. Three different DNA detection schemes are described: (i) analysis of a 27-base nucleic acid fragment; (ii) analysis of the entire M13phi DNA (7229 bases); and (iii) detection of a single-base mismatch in a DNA. Ultrasensitive detection of DNA is accomplished by the catalyzed deposition of gold, detection limit approximately 1 x 10(-15) M.
ISSN:0003-2654
DOI:10.1039/b106613g