Disassembly of Melanosomes in Detergents

A method is reported for the isolation of melanosomes from black mouse hair. Keratin is removed from the hair by a two-step process consisting of alkaline hydrolysis and subsequent enzymatic (trypsin) digestion. The effects upon isolated melanosomes of several protein denaturing agents such as urea,...

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Bibliographic Details
Published in:	Journal of investigative dermatology Vol. 60; no. 4; pp. 224 - 230
Main Author:	Bratosin, Suzana
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-04-1973
Subjects:	Animals Chromatography Chromatography, Gel Detergents - pharmacology Hair - analysis Immunoglobulins - analysis Keratins - analysis Melanocytes - analysis Melanocytes - cytology Melanocytes - drug effects Mercaptoethanol - pharmacology Methods Mice Microscopy, Electron Molecular Weight Pigments, Biological - isolation & purification Proteins - analysis Urea - pharmacology
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Summary:	A method is reported for the isolation of melanosomes from black mouse hair. Keratin is removed from the hair by a two-step process consisting of alkaline hydrolysis and subsequent enzymatic (trypsin) digestion. The effects upon isolated melanosomes of several protein denaturing agents such as urea, guanidine hydrochloride, 2-mercaptoethanol, and various detergents have been studied at several pHs and temperatures. It was found that detergents caused dissolution of melanosomes yielding three major melanoproteins which differ in electrophoretic mobility and molecular weight.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-202X 1523-1747
DOI:	10.1111/1523-1747.ep12724518