Early and Effective Diagnosis of Sepsis Using Flow Cytometry
Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According...
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| Published in: | Journal of laboratory physicians Vol. 15; no. 2; pp. 230 - 236 |
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01-06-2023
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| Abstract | Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center.
Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups.
The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19-100%) and 100% (95% CI: 55.32-86.83%); specificity of 90.00% (95% CI: 59.58-99.49%) and 87.24% (95% CI: 66.69-99.61%); and likelihood ratio of 10.00 and 7.84, respectively.
The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients. |
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| AbstractList | Objective
Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center.
Materials and Methods
Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups.
Results
The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19–100%) and 100% (95% CI: 55.32–86.83%); specificity of 90.00% (95% CI: 59.58–99.49%) and 87.24% (95% CI: 66.69–99.61%); and likelihood ratio of 10.00 and 7.84, respectively.
Conclusion
The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients. Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center. Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups. The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19-100%) and 100% (95% CI: 55.32-86.83%); specificity of 90.00% (95% CI: 59.58-99.49%) and 87.24% (95% CI: 66.69-99.61%); and likelihood ratio of 10.00 and 7.84, respectively. The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients. Objective Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center. Materials and Methods Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups. Results The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19–100%) and 100% (95% CI: 55.32–86.83%); specificity of 90.00% (95% CI: 59.58–99.49%) and 87.24% (95% CI: 66.69–99.61%); and likelihood ratio of 10.00 and 7.84, respectively. Conclusion The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients. Objective Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center. Materials and Methods Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups. Results The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19–100%) and 100% (95% CI: 55.32–86.83%); specificity of 90.00% (95% CI: 59.58–99.49%) and 87.24% (95% CI: 66.69–99.61%); and likelihood ratio of 10.00 and 7.84, respectively. Conclusion The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients. |
| Author | Kushwaha, Rashmi Verma, Shailendra P Agarwal, Avinash Yadav, Geeta Singh, Uma S Singh, Anurag Verma, Pratima Reddy, Himanshu D |
| AuthorAffiliation | 2 Department of Clinical Hematology, King George's Medical University, Lucknow, Uttar Pradesh, India 4 Department of Critical Care Medicine, King George's Medical University, Lucknow, Uttar Pradesh, India 1 Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India 3 Department of Medicine, King George's Medical University, Lucknow, Uttar Pradesh, India |
| AuthorAffiliation_xml | – name: 1 Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India – name: 4 Department of Critical Care Medicine, King George's Medical University, Lucknow, Uttar Pradesh, India – name: 3 Department of Medicine, King George's Medical University, Lucknow, Uttar Pradesh, India – name: 2 Department of Clinical Hematology, King George's Medical University, Lucknow, Uttar Pradesh, India |
| Author_xml | – sequence: 1 givenname: Pratima surname: Verma fullname: Verma, Pratima organization: Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India – sequence: 2 givenname: Anurag surname: Singh fullname: Singh, Anurag organization: Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India – sequence: 3 givenname: Rashmi orcidid: 0000-0002-0581-2177 surname: Kushwaha fullname: Kushwaha, Rashmi organization: Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India – sequence: 4 givenname: Geeta surname: Yadav fullname: Yadav, Geeta organization: Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India – sequence: 5 givenname: Shailendra P surname: Verma fullname: Verma, Shailendra P organization: Department of Clinical Hematology, King George's Medical University, Lucknow, Uttar Pradesh, India – sequence: 6 givenname: Uma S surname: Singh fullname: Singh, Uma S organization: Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India – sequence: 7 givenname: Himanshu D surname: Reddy fullname: Reddy, Himanshu D organization: Department of Medicine, King George's Medical University, Lucknow, Uttar Pradesh, India – sequence: 8 givenname: Avinash surname: Agarwal fullname: Agarwal, Avinash organization: Department of Critical Care Medicine, King George's Medical University, Lucknow, Uttar Pradesh, India |
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| Cites_doi | 10.1001/jama.2009.1754 10.1515/CCLM.2009.224 10.1016/j.pcl.2004.03.009 10.1177/0885066608327095 10.5858/2006-130-654-NCIAII 10.1016/j.bios.2018.10.034 10.1128/CMR.00089-17 10.1165/rcmb.2008-0252OC 10.1093/cid/cit936 10.1186/1757-7241-17-67 10.1186/s13054-015-0972-z 10.1155/2008/202646 10.1111/j.1440-1843.2010.01876.x 10.1007/s00134-012-2483-6 10.22159/ajpcr.2017.v10i11.20640 10.1097/00003246-200202000-00001 10.1016/j.talanta.2018.08.058 10.1136/bmj.39346.495880.AE 10.1128/JCM.00628-09 10.15585/mmwr.mm6533e1 10.1111/j.1365-2249.2011.04380.x 10.1016/S0140-6736(19)32989-7 10.4103/jgid.jgid_130_16 10.1007/s10156-008-0608-1 10.1590/S0021-75572003000800009 10.1016/j.jinf.2010.02.013 10.1016/S0140-6736(04)17667-8 10.4103/2229-5151.141356 |
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| Snippet | Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to... Objective Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it... Objective Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it... |
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| SubjectTerms | biomarker cd64 flow cytometry intensive care unit Original sepsis |
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| Title | Early and Effective Diagnosis of Sepsis Using Flow Cytometry |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/37323597 https://search.proquest.com/docview/2827254639 https://pubmed.ncbi.nlm.nih.gov/PMC10264123 https://doaj.org/article/c94375a7481240d09d6a67e24506c36b |
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