In vitro selection of yellow passion fruit genotypes for resistance to Fusarium vascular wilt

• Published in: Plant cell, tissue and organ culture Vol. 108; no. 1; pp. 37 - 45
• Main Authors: Flores, Patrícia Silva, Otoni, Wagner Campos, Dhingra, Onkar Dev, de Souza Diniz, Sérgio Paulo Severo, Santos, Telma Miranda dos, Bruckner, Claudio Horst
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-01-2012; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cultivars; Cultivation; Culture media; Filtrate; Fruits; Fungi; Fusaric acid; Fusarium; Fusarium oxysporum; Genotypes; Life Sciences; Original Paper; Passion fruit; Pathogens; Plant Genetics and Genomics; Plant growth; Plant Pathology; Plant Physiology; Plant Sciences; Plantlets; Seeds; Sensitivity analysis; Suspension culture; Toxins; Wilt; Culture filtrate; Tissue culture; f. sp; Fusaric acid
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-011-0009-5

Fusarium
vascular wilt (caused by
Fusarium oxysporum
f. sp.
passiflorae
) is a limiting factor in the cultivation of yellow passion fruit (
Passiflora edulis
). Since there is no effective and economically viable control available, development of resistant or at least tolerant cultivars are in demand. A number of procedures have been used for the initial selection of plant genotypes resistant to various fungal pathogens by means of a fungal culture filtrate or purified toxin. In this study, seeds and in vitro-grown plantlets of passion fruit were screened with different concentrations of either
Fusarium oxysporum
f. sp.
passiflorae
(FOP) culture filtrate (0, 20, 30, 40 or 50%, v/v) or fusaric acid (0.10, 0.20, 0.30 or 0.40 mM) supplemented in Murashige and Skoog (MS) basal media. Subsequently, selected plants were inoculated with a conidial suspension of FOP to assess correlation between in vivo and in vitro responses. In vitro sensitivity to the selective agents and the resistance response to the pathogen were also compared. Root growth was markedly influenced by FA, culture filtrate, and conidial suspension culture treatments. Observations indicated that roots were primary targets for attack by
F. oxysporum
. Successful in vitro selection of resistant genotypes by both FA and culture filtrate treatments suggested that this strategy was viable for accelerating breeding of passion fruit for resistance to the
Fusarium
vascular wilt.