Isolation of nine microsatellite loci in Dolichogenidea homoeosomae (Hymenoptera) a parasitoid of the sunflower moth Homoeosoma electellum (Lepidoptera)

• Published in: Molecular ecology notes Vol. 6; no. 1; pp. 96 - 97
• Main Authors: Douhovnikoff, Vladimir, Nerney, Caterina, Roderick, George K, Newton, Craig H, Welter, Stephen C
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Science Ltd 01-03-2006; Blackwell Science Ltd
• Subjects: alleles; alleles per locus; biological control agents; DNA primers; Dolichogenidea; Dolichogenidea homoeosomae; genetic markers; heterozygosity; Homosoeosoma; Hymenoptera microsatellites; loci; microsatellite repeats; molecular sequence data; nucleotide sequences; parasitoid; parasitoids; polymerase chain reaction; polymorphic loci; population structure; California
• ISSN: 1471-8278; 1471-8286
• DOI: 10.1111/j.1471-8286.2005.01151.x

Nine microsatellite loci were isolated from the insect Dolichogenidea homoeosomae (Hymenoptera: Braconidae), an important parasitoid of the sunflower moth Homosoeosoma electellum (Lepidoptera: Pyralidae), and assayed for polymorphism. All nine loci were polymorphic within the five populations tested, with two to 14 alleles per locus. Expected and observed heterozygosities ranged from 0.39 to 0.90 and 0.25 to 0.72 respectively. These are the first microsatellite primers developed for D. homeosomae and will be useful for studies of population dynamics and connectivity.