Isolation of a Population of Cells Co-Expressing Markers of Embryonic Stem Cells and Mesenchymal Stem Cells from the Rudimentary Uterine Horn of a Patient with Uterine Aplasia

• Published in: Bulletin of experimental biology and medicine Vol. 174; no. 4; pp. 549 - 555
• Main Authors: Burunova, V. V., Gisina, A. M., Yarygina, N. K., Sukhinich, K. K., Makiyan, Z. N., Yarygin, K. N.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-02-2023; Springer; Springer Nature B.V
• Subjects: Antibodies; Aplasia; Biology; Biomedical and Life Sciences; Biomedicine; Blood; Bone marrow; Cell Biology; Cell Differentiation - physiology; Cell membranes; Embryo cells; Embryogenesis; Embryonic stem cells; Embryonic Stem Cells - metabolism; Endometrium; Endometrium - metabolism; Ethylenediaminetetraacetic acid; Female; Flow cytometry; Gynecology; Humans; Internal Medicine; Laboratory Medicine; Medical research; Mesenchymal stem cells; Mesenchymal Stem Cells - metabolism; Morphogenesis; Myometrium; Obstetrics; Oct-4 protein; Pathology; Stem cells; Transcription factors; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); Uterus; Uterus - metabolism; Uterus - pathology; SSEA4; Oct4; Nanog; postnatal stem cells; histogenesis
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-023-05746-w

More than 50% cells isolated from the endometrial cavity scraping and the myometrium of the rudimentary horn of an underdeveloped uterus removed from a patient with uterine aplasia and maintained under culturing conditions normal for mesenchymal stem cells (MSC) expressed embryonic transcription factors Oct4 and Nanog, embryonic cell membrane sialyl glycolipid SSEA4, and MSC markers. After 2-3 passages, the cells lost the expression of the early embryogenesis markers, but retained MSC markers. The presence of dormant stem cells in the underdeveloped endometrium and in the uterus indicates that this tissue has a regenerative potential that can be activated and used for completion of organ morphogenesis. This task requires the development of methods of early diagnosis of morphogenesis impairment and tools for safe reactivation of the ontogenesis.