Cucumibacter marinus gen. nov., sp. nov., a marine bacterium in the family Hyphomicrobiaceae

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 7; pp. 1591 - 1597
• Main Authors: Hwang, Chung Y, Cho, Byung C
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-07-2008; Society for General Microbiology
• Subjects: Bacteria; Bacteriology; Biological and medical sciences; Cucumibacter marinus; Devosia; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Hyphomicrobiaceae - chemistry; Hyphomicrobiaceae - classification; Hyphomicrobiaceae - genetics; Hyphomicrobiaceae - ultrastructure; Korea; Marine; Microbiology; Microscopy, Electron, Transmission; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Species Specificity; Systematics; Korea; INW, Korea, Rep; Marine environment; New genus; Taxonomy; Bacteria; New species; Hyphomicrobiaceae; Hyphomicrobiales
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65587-0

School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea
Correspondence Byung C. Cho bccho{at}snu.ac.kr
A Gram-negative, rod-shaped, strictly aerobic micro-organism, designated strain CL-GR60 T , was isolated from coastal seawater from the East Sea, Korea. A 16S rRNA gene sequence analysis revealed a clear affiliation with the family Hyphomicrobiaceae . Phylogenetic analyses showed that strain CL-GR60 T formed a robust cluster with the species of the genus Devosia at sequence similarity levels of 91.1–93.1 %; no other species in the family Hyphomicrobiaceae shared more than 90 % sequence similarity with strain CL-GR60 T . The strain grew optimally in the presence of 3–4 % sea salts at 30–35 °C and pH 7. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, two unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The strain contained C 18 : 1 7 c (54.7 %), 11-methyl C 18 : 1 7 c (19.3 %), C 18 : 0 (14.4 %) as the predominant fatty acids. Ubiquinone-10 was the major quinone. The DNA G+C content was 62.9 mol%. On the basis of physiological data, fatty acid composition and results of phylogenetic analysis of the 16S rRNA gene sequence, strain CL-GR60 T represents a novel genus and species within the family Hyphomicrobiaceae , for which the name Cucumibacter marinus gen. nov., sp. nov. is proposed. The type strain of Cucumibacter marinus is CL-GR60 T (=KCCM 90027 T =DSM 18995 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CL-GR60 T is EF211830 .