Marinobacter salsuginis sp. nov., isolated from the brine-seawater interface of the Shaban Deep, Red Sea

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 5; pp. 1035 - 1040
• Main Authors: Antunes, Andre, Franca, Luis, Rainey, Fred A, Huber, Robert, Nobre, M. Fernanda, Edwards, Katrina J, da Costa, Milton S
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-05-2007; Society for General Microbiology
• Subjects: Anaerobiosis; Animal, plant and microbial ecology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Carbohydrate Metabolism; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Flagella; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Hydrogen-Ion Concentration; Indian Ocean; Marine; Marinobacter; Marinobacter - classification; Marinobacter - genetics; Marinobacter - isolation & purification; Marinobacter - physiology; Marinobacter salsuginis; Microbial ecology; Microbiology; Molecular Sequence Data; Movement; Nitrates - metabolism; Nitrogen - metabolism; Oxidation-Reduction; Phylogeny; Pigments, Biological - biosynthesis; Quinones - analysis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sodium Chloride - metabolism; Systematics; Temperature; Various environments (extraatmospheric space, air, water); Red Sea; Indian Ocean; ISW, Red Sea, Shaban Deep; Brackish water environment; Bacteria; New species; Taxonomy; Seawater
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64862-0

1 Laboratório de Microbiologia, Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal
2 Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA
3 Lehrstuhl für Mikrobiologie und Archaeenzentrum, Universität Regensburg, D-93053 Regensburg, Germany
4 Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal
5 Department of Biological Sciences, Division of Marine & Environmental Biology, University of Southern California, Los Angeles, CA 90089-0271, USA
6 Departamento de Bioquímica, Universidade de Coimbra, 3001-401 Coimbra, Portugal
Correspondence Milton S. da Costa milton{at}ci.uc.pt
Two moderately halophilic Gram-negative bacteria were isolated from a sample taken from the brine–seawater interface of the Shaban Deep in the Red Sea. Phylogenetic analysis of the 16S rRNA gene sequence showed that these organisms represent a novel species of the genus Marinobacter . Cells of the new isolates formed non-pigmented colonies and were motile by means of a single polar flagellum. Strains SD-14B T and SD-14C grew optimally at 35–37 °C, in 5 % NaCl and at pH 7.5–8.0. The organisms were aerobic, but reduced nitrate to nitrogen under anaerobic conditions. Acid was produced from only a few carbohydrates. Ubiquinone 9 was the major respiratory quinone. The major fatty acids of strains SD-14B T and SD-14C were C 16 : 0 , C 18 : 1 9 c , summed feature 3 (C 16 : 1 6 c /C 16 : 1 7 c ) and C 12 : 0 3-OH. The DNA G+C contents were 55.9 and 55.7 mol%, respectively. On the basis of the phylogenetic analyses and physiological and biochemical characteristics, it is proposed that strains SD-14B T and SD-14C represent a novel species of the genus Marinobacter , with the name Marinobacter salsuginis sp. nov. The type strain is strain SD-14B T (=DSM 18347 T =LMG 23697 T ).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SD-14B T is EF028328 .