Single-spore elemental analyses indicate that dipicolinic acid-deficient Bacillus subtilis spores fail to accumulate calcium

• Published in: Archives of microbiology Vol. 192; no. 6; pp. 493 - 497
• Main Authors: Hintze, Paul E, Nicholson, Wayne L
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-06-2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Acids; Bacillus subtilis; Bacillus subtilis - chemistry; Bacillus subtilis - metabolism; Bacillus subtilis - ultrastructure; Bacteriology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Calcium; Calcium - analysis; Calcium - metabolism; Carboxylic acids; Cell Biology; Dipicolinic acid; DPA; Ecology; Fundamental and applied biological sciences. Psychology; Heat resistance; Life Sciences; Microbial Ecology; Microbiology; Microscopy, Electron, Scanning; Miscellaneous; Picolinic Acids - analysis; Picolinic Acids - metabolism; Proteins; RNA polymerase; Short Communication; Spectrometry, X-Ray Emission; spores; Spores, Bacterial - chemistry; Spores, Bacterial - ultrastructure; Temperature; X-ray spectroscopy; United States > US; Dipicolinic acid; DPA; Spore; Bacillales; Calcium; Bacillus subtilis; Bacteria; Bacillaceae; Inorganic element; Spores
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-010-0569-5

Dipicolinic acid (pyridine-2,6-carboxylic acid; DPA) is a major component of bacterial spores and has been shown to be an important determinant of spore resistance. In the core of dormant Bacillus subtilis spores, DPA is associated with divalent calcium in a 1:1 chelate (Ca-DPA). Spores excrete Ca-DPA during germination, but it is unknown whether Ca and DPA are imported separately or together into the developing spore. Elemental analysis by scanning electron microscopy-energy-dispersive X-ray spectroscopy (SEM-EDS) of wild-type spores and mutant spores lacking the ability to synthesize DPA showed that DPA-less spores also lacked calcium, suggesting that the two compounds may be co-imported.