Ovariectomy induces osteoporosis in the maxillary alveolar bone: an in vivo micro-CT and histomorphometric analysis in rats

Objective To investigate whether porotic changes occur in maxillary alveolar bone in ovariectomized rats. Methods Thirty‐two 6‐week‐old female Sprague‐Dawley rats were divided into an ovariectomy group (OVX) and a sham‐ovariectomy group (sham). Twelve weeks after surgery, maxillae of 16 rats (eight...

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Published in:Oral diseases Vol. 20; no. 5; pp. 514 - 520
Main Authors: Dai, Q-G, Zhang, P, Wu, Y-Q, Ma, X-H, Pang, J, Jiang, L-Y, Fang, B
Format: Journal Article
Language:English
Published: Denmark Blackwell Publishing Ltd 01-07-2014
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Summary:Objective To investigate whether porotic changes occur in maxillary alveolar bone in ovariectomized rats. Methods Thirty‐two 6‐week‐old female Sprague‐Dawley rats were divided into an ovariectomy group (OVX) and a sham‐ovariectomy group (sham). Twelve weeks after surgery, maxillae of 16 rats (eight OVX and eight sham) were analyzed by micro‐CT. Inter‐radicular alveolar bone of the maxillary first molar was reconstructed and analyzed. The remaining 16 maxillae were used for histochemistry, including hematoxylin‐eosin staining and tartrate‐resistant acid phosphatase enzyme staining. Results Three‐dimensional reconstructed images of the irregular alveolar bone showed an intuitive view of porotic changes in the OVX group. The alveolar bone in OVX rats had a porous microarchitecture including lower bone mass and a looser structure of more widely separated trabeculae. Bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular thickness (Tb.Th.) were significantly lower in the OVX group than those in the sham group (P < 0.05). Trabecular separation (Tb.Sp.) increased significantly in the OVX rats compared to the sham rats (P < 0.05). Histomorphometric analysis of alveolar bone also revealed porotic changes in the OVX rats. Bone area ratio significantly decreased in the OVX group compared with the sham group (P < 0.01). There were also more osteoclasts present in the alveolar bone of OVX rats compared to sham rats (P < 0.05). Conclusion Ovariectomy induces osteoporosis in maxillary alveolar bone in rats, which may be related to the increased number of osteoclasts.
Bibliography:Collaborative Foundation of Medical and Engineering Science of Shanghai Jiaotong University - No. YG2012MS40
ark:/67375/WNG-CNCC92ZM-D
ArticleID:ODI12166
Key Basic Research Foundation of the Shanghai Committee of Science and Technology - No. 12JC1405700
Natural Science Foundation of Shanghai - No. 13ZR1423700
istex:59A636D7CE7591156D030BA4B2ACA9F8C7C7E24F
National Natural Science Foundation of China - No. 30901698; No. 10972142
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ISSN:1354-523X
1601-0825
DOI:10.1111/odi.12166