Ovariectomy induces osteoporosis in the maxillary alveolar bone: an in vivo micro-CT and histomorphometric analysis in rats
Objective To investigate whether porotic changes occur in maxillary alveolar bone in ovariectomized rats. Methods Thirty‐two 6‐week‐old female Sprague‐Dawley rats were divided into an ovariectomy group (OVX) and a sham‐ovariectomy group (sham). Twelve weeks after surgery, maxillae of 16 rats (eight...
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Published in: | Oral diseases Vol. 20; no. 5; pp. 514 - 520 |
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Main Authors: | , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Denmark
Blackwell Publishing Ltd
01-07-2014
Wiley Subscription Services, Inc |
Subjects: | |
Online Access: | Get full text |
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Summary: | Objective
To investigate whether porotic changes occur in maxillary alveolar bone in ovariectomized rats.
Methods
Thirty‐two 6‐week‐old female Sprague‐Dawley rats were divided into an ovariectomy group (OVX) and a sham‐ovariectomy group (sham). Twelve weeks after surgery, maxillae of 16 rats (eight OVX and eight sham) were analyzed by micro‐CT. Inter‐radicular alveolar bone of the maxillary first molar was reconstructed and analyzed. The remaining 16 maxillae were used for histochemistry, including hematoxylin‐eosin staining and tartrate‐resistant acid phosphatase enzyme staining.
Results
Three‐dimensional reconstructed images of the irregular alveolar bone showed an intuitive view of porotic changes in the OVX group. The alveolar bone in OVX rats had a porous microarchitecture including lower bone mass and a looser structure of more widely separated trabeculae. Bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular thickness (Tb.Th.) were significantly lower in the OVX group than those in the sham group (P < 0.05). Trabecular separation (Tb.Sp.) increased significantly in the OVX rats compared to the sham rats (P < 0.05). Histomorphometric analysis of alveolar bone also revealed porotic changes in the OVX rats. Bone area ratio significantly decreased in the OVX group compared with the sham group (P < 0.01). There were also more osteoclasts present in the alveolar bone of OVX rats compared to sham rats (P < 0.05).
Conclusion
Ovariectomy induces osteoporosis in maxillary alveolar bone in rats, which may be related to the increased number of osteoclasts. |
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Bibliography: | Collaborative Foundation of Medical and Engineering Science of Shanghai Jiaotong University - No. YG2012MS40 ark:/67375/WNG-CNCC92ZM-D ArticleID:ODI12166 Key Basic Research Foundation of the Shanghai Committee of Science and Technology - No. 12JC1405700 Natural Science Foundation of Shanghai - No. 13ZR1423700 istex:59A636D7CE7591156D030BA4B2ACA9F8C7C7E24F National Natural Science Foundation of China - No. 30901698; No. 10972142 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1354-523X 1601-0825 |
DOI: | 10.1111/odi.12166 |