The development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis

Summary Accurate, rapid and economical fungal species identification has been a major aim in mycology. In this study, our goal was to examine the feasibility of a high‐resolution melting curve analysis (HRMA) of internal transcribed regions ITS1 and ITS2 in ribosomal DNA (rDNA) for a rapid, simple a...

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Bibliographic Details
Published in:	Molecular oral microbiology Vol. 29; no. 3; pp. 117 - 130
Main Authors:	Alnuaimi, A.D., Wiesenfeld, D., O'Brien-Simpson, N.M., Reynolds, E.C., Peng, B., McCullough, M.J.
Format:	Journal Article
Language:	English
Published:	Denmark Blackwell Publishing Ltd 01-06-2014 Wiley Subscription Services, Inc
Subjects:	Candida - classification Candida - genetics Candida albicans Candida dubliniensis Candida genotyping Candida glabrata Candida guilliermondii Candida krusei Candida lusitaniae Candida parapsilosis Candida tropicalis Dentistry DNA, Fungal - chemistry DNA, Ribosomal - chemistry Feasibility Studies Genotype high-resolution melting curve analysis Humans Nucleic Acid Denaturation rapid identification Real-Time Polymerase Chain Reaction Species Specificity Candida albicans high-resolution melting curve analysis rapid identification Candida genotyping
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