Reduced PMA enhances the responsiveness of transfected THP-1 macrophages to polarizing stimuli

Macrophages are versatile cells of the immune system which react to various external stimuli through different polarization patterns which adjust the cells to the required function whether it is removal of pathogens or necrotic cells, tissue repair or propagation of inflammation. As much of macropha...

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Bibliographic Details
Published in:	Journal of immunological methods Vol. 402; no. 1-2; pp. 76 - 81
Main Authors:	Maeß, Marten B., Wittig, Berith, Cignarella, Andrea, Lorkowski, Stefan
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 15-01-2014
Subjects:	Activation Antigens, CD - genetics Antigens, CD - metabolism Antigens, Differentiation, Myelomonocytic - genetics Antigens, Differentiation, Myelomonocytic - metabolism Apoptosis - drug effects CD11b Antigen - genetics CD11b Antigen - metabolism Cell Line Cell Shape - drug effects Cell Survival - drug effects Chemokine CCL3 - genetics Chemokine CCL3 - metabolism Dose-Response Relationship, Drug Humans IL10 Interferon-gamma - pharmacology Interleukin-10 - pharmacology Interleukin-10 Receptor beta Subunit - genetics Interleukin-10 Receptor beta Subunit - metabolism Lipopolysaccharides - pharmacology Macrophage Activation - drug effects Macrophages - drug effects Macrophages - immunology Macrophages - metabolism Necrosis Polarization Receptors, CCR7 - genetics Receptors, CCR7 - metabolism RNA Interference RNA, Small Interfering - metabolism siRNA Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins - genetics Suppressor of Cytokine Signaling Proteins - metabolism Tetradecanoylphorbol Acetate - pharmacology THP-1 macrophages Transfection Transfection - methods Tumor Necrosis Factor-alpha - metabolism Up-Regulation IFNγ IL10 Polarization TNFα IL IL10RB PMA PKC siRNA Activation LPS SOCS3 7-AAD TGFβ1 Transfection PE FCS RPL37A CCL3 CCR7 THP-1 macrophages RT-qPCR suppressor of cytokine signaling 3 tumor growth factor β1 tumor necrosis factor α chemokine (C–C motif) ligand 3 phorbol 12-myristate 13-acetate reverse transcriptase quantitative polymerase chain reaction interferon γ fetal calf serum interleukin protein kinase C chemokine (C–C motif) receptor 7 7-aminoactinomycin phycoerythrin interleukin 10 receptor β chain lipopolysaccharide ribosomal protein L37A
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