Gld2-catalyzed 3' monoadenylation of miRNAs in the hippocampus has no detectable effect on their stability or on animal behavior

Gld2, a noncanonical cytoplasmic poly(A) polymerase, interacts with the RNA binding protein CPEB1 to mediate polyadenylation-induced translation in dendrites of cultured hippocampal neurons. Depletion of Gld2 from the hippocampus leads to a deficit in long-term potentiation evoked by theta burst sti...

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Bibliographic Details
Published in:	RNA (Cambridge) Vol. 22; no. 10; pp. 1492 - 1499
Main Authors:	Mansur, Fernanda, Ivshina, Maria, Gu, Weifeng, Schaevitz, Laura, Stackpole, Emily, Gujja, Sharvari, Edwards, Yvonne J K, Richter, Joel D
Format:	Journal Article
Language:	English
Published:	United States Cold Spring Harbor Laboratory Press 01-10-2016
Subjects:	Animals Behavior, Animal Hippocampus - metabolism Hippocampus - physiology Male Mice Mice, Inbred C57BL MicroRNAs - genetics MicroRNAs - metabolism Polynucleotide Adenylyltransferase - genetics Polynucleotide Adenylyltransferase - metabolism RNA 3' End Processing RNA Stability miRNA Gld2 hippocampus behavior monoadenylation
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Summary:	Gld2, a noncanonical cytoplasmic poly(A) polymerase, interacts with the RNA binding protein CPEB1 to mediate polyadenylation-induced translation in dendrites of cultured hippocampal neurons. Depletion of Gld2 from the hippocampus leads to a deficit in long-term potentiation evoked by theta burst stimulation. At least in mouse liver and human primary fibroblasts, Gld2 also 3' monoadenylates and thereby stabilizes specific miRNAs, which enhance mRNA translational silencing and eventual destruction. These results suggest that Gld2 would be likely to monoadenylate and stabilize miRNAs in the hippocampus, which would produce measurable changes in animal behavior. We now report that using Gld2 knockout mice, there are detectable alterations in specific miRNA monoadenylation in the hippocampus when compared to wild type, but that these modifications produce no detectable effect on miRNA stability. Moreover, we surprisingly find no overt change in animal behavior when comparing Gld2 knockout to wild-type mice. These data indicate that miRNA monoadenylation-mediated stability is cell type-specific and that monoadenylation has no measurable effect on higher cognitive function.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	1355-8382 1469-9001
DOI:	10.1261/rna.056937.116