Label-free sensing of thrombin based on quantum dots and thrombin binding aptamer

A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The crude QDs can be “activated” with fluorescence enhancement by adding extra Cd2+ to the solution in basic medium. As a result, the positively char...

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Published in:	Talanta (Oxford) Vol. 107; pp. 140 - 145
Main Authors:	Zhang, Xiangyuan, Hu, Ruoxin, Shao, Na
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 30-03-2013
Subjects:	Aptamers, Nucleotide - chemistry Binding cadmium Cadmium - chemistry Cadmium Compounds - chemistry Cadmium tellurides CdTe quantum dots Charging Detection detection limit electrostatic interactions Fluorescence Fluorescence recovery Fluorescent Dyes - chemistry G-Quadruplexes Humans Label-free Limit of Detection oligonucleotides Quantum Dots Quenching Spectrometry, Fluorescence - methods Tellurium - chemistry Thrombin Thrombin - analysis Thrombin binding aptamer Thrombin binding aptamer Fluorescence recovery Thrombin CdTe quantum dots Label-free
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Abstract	A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The crude QDs can be “activated” with fluorescence enhancement by adding extra Cd2+ to the solution in basic medium. As a result, the positively charged Cd2+-activating CdTe QDs could interact with the negatively charged TBA, leading to fluorescence quenching. When thrombin was added, TBA was induced to form a G-quadruplex structure and combined specifically with its target, releasing the QDs with a recovery of the fluorescence intensity. The sensing approach is based on the strongly specific interactions between TBA and thrombin over the electrostatic interactions between TBA and positively charged QDs. Based on the fluorescence enhancement of QDs, selective detection of thrombin was successfully achieved. A linear response for thrombin was observed in the range from 1.4nM to 21nM with a detection limit of 0.70nM. ► Label-free aptamer based detection of thrombin is realized using CdTe QDs. ► Easy to operate as the complicated cross-linking reactions were eliminated. ► Turn-on sensing mode could reduce background signal and improve sensitivity. ► Serum sample analysis was achieved, requiring no pretreatment or separation. ► This work may offer a relatively general approach to other analytes detection.
AbstractList	A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The crude QDs can be "activated" with fluorescence enhancement by adding extra Cd(2+) to the solution in basic medium. As a result, the positively charged Cd(2+)-activating CdTe QDs could interact with the negatively charged TBA, leading to fluorescence quenching. When thrombin was added, TBA was induced to form a G-quadruplex structure and combined specifically with its target, releasing the QDs with a recovery of the fluorescence intensity. The sensing approach is based on the strongly specific interactions between TBA and thrombin over the electrostatic interactions between TBA and positively charged QDs. Based on the fluorescence enhancement of QDs, selective detection of thrombin was successfully achieved. A linear response for thrombin was observed in the range from 1.4 nM to 21 nM with a detection limit of 0.70 nM. A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The crude QDs can be aactivateda with fluorescence enhancement by adding extra Cd2+ to the solution in basic medium. As a result, the positively charged Cd2+-activating CdTe QDs could interact with the negatively charged TBA, leading to fluorescence quenching. When thrombin was added, TBA was induced to form a G-quadruplex structure and combined specifically with its target, releasing the QDs with a recovery of the fluorescence intensity. The sensing approach is based on the strongly specific interactions between TBA and thrombin over the electrostatic interactions between TBA and positively charged QDs. Based on the fluorescence enhancement of QDs, selective detection of thrombin was successfully achieved. A linear response for thrombin was observed in the range from 1.4 nM to 21 nM with a detection limit of 0.70 nM. A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The crude QDs can be “activated” with fluorescence enhancement by adding extra Cd2+ to the solution in basic medium. As a result, the positively charged Cd2+-activating CdTe QDs could interact with the negatively charged TBA, leading to fluorescence quenching. When thrombin was added, TBA was induced to form a G-quadruplex structure and combined specifically with its target, releasing the QDs with a recovery of the fluorescence intensity. The sensing approach is based on the strongly specific interactions between TBA and thrombin over the electrostatic interactions between TBA and positively charged QDs. Based on the fluorescence enhancement of QDs, selective detection of thrombin was successfully achieved. A linear response for thrombin was observed in the range from 1.4nM to 21nM with a detection limit of 0.70nM. ► Label-free aptamer based detection of thrombin is realized using CdTe QDs. ► Easy to operate as the complicated cross-linking reactions were eliminated. ► Turn-on sensing mode could reduce background signal and improve sensitivity. ► Serum sample analysis was achieved, requiring no pretreatment or separation. ► This work may offer a relatively general approach to other analytes detection. A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The crude QDs can be “activated” with fluorescence enhancement by adding extra Cd²⁺ to the solution in basic medium. As a result, the positively charged Cd²⁺-activating CdTe QDs could interact with the negatively charged TBA, leading to fluorescence quenching. When thrombin was added, TBA was induced to form a G-quadruplex structure and combined specifically with its target, releasing the QDs with a recovery of the fluorescence intensity. The sensing approach is based on the strongly specific interactions between TBA and thrombin over the electrostatic interactions between TBA and positively charged QDs. Based on the fluorescence enhancement of QDs, selective detection of thrombin was successfully achieved. A linear response for thrombin was observed in the range from 1.4nM to 21nM with a detection limit of 0.70nM.
Author	Zhang, Xiangyuan Shao, Na Hu, Ruoxin
Author_xml	– sequence: 1 givenname: Xiangyuan surname: Zhang fullname: Zhang, Xiangyuan – sequence: 2 givenname: Ruoxin surname: Hu fullname: Hu, Ruoxin – sequence: 3 givenname: Na surname: Shao fullname: Shao, Na email: shaona@bnu.edu.cn
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/23598204$$D View this record in MEDLINE/PubMed
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Keywords	Thrombin binding aptamer Fluorescence recovery Thrombin CdTe quantum dots Label-free
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Snippet	A facile and sensitive label-free approach for detection of thrombin based on CdTe quantum dots (QDs) and thrombin binding aptamer (TBA) is presented. The...
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SubjectTerms	Aptamers, Nucleotide - chemistry Binding cadmium Cadmium - chemistry Cadmium Compounds - chemistry Cadmium tellurides CdTe quantum dots Charging Detection detection limit electrostatic interactions Fluorescence Fluorescence recovery Fluorescent Dyes - chemistry G-Quadruplexes Humans Label-free Limit of Detection oligonucleotides Quantum Dots Quenching Spectrometry, Fluorescence - methods Tellurium - chemistry Thrombin Thrombin - analysis Thrombin binding aptamer
Title	Label-free sensing of thrombin based on quantum dots and thrombin binding aptamer
URI	https://dx.doi.org/10.1016/j.talanta.2013.01.003 https://www.ncbi.nlm.nih.gov/pubmed/23598204 https://search.proquest.com/docview/1331088633 https://search.proquest.com/docview/1513421461
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