A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood

• Published in: Biochemia Medica Vol. 27; no. 2; pp. 350 - 377
• Main Authors: Ozarda, Yesim, Ichihara, Kiyoshi, Bakan, Ebubekir, Polat, Harun, Ozturk, Nurinnisa, Baygutalp, Nurcan K, Taneli, Fatma, Guvenc, Yesim, Ormen, Murat, Erbayraktar, Zubeyde, Aksoy, Nurten, Sezen, Hatice, Demir, Meltem, Eskandari, Gulcin, Polat, Gurbuz, Mete, Nuriye, Yuksel, Hatice, Vatansev, Husamettin, Gun, Fatma, Akin, Okhan, Ceylan, Ozlem, Noyan, Tevfik, Gozlukaya, Ozgul, Aliyazicioglu, Yuksel, Kahraman, Sevim, Dirican, Melahat, Tuncer, Gul Ozlem, Kimura, Shogo, Eker, Pinar
• Format: Journal Article Paper
• Language: English
• Published: Croatia Medicinska naklada 15-06-2017; Croatian Society of Medical Biochemistry and Laboratory Medicine
• Subjects: Adolescent; Adult; Aged; Blood Cell Count; complete blood count; Female; haematology; Hematologic Tests - instrumentation; Hematologic Tests - methods; Hematologic Tests - standards; Humans; Laboratories - standards; Laboratory Proficiency Testing - standards; Male; Middle Aged; multicentre study; Original Papers; Quality Control; reference intervals; Reference Values; Regression Analysis; Reproducibility of Results; Turkey; Young Adult; haematology; reference intervals; Turkey; multicentre study; complete blood count
• DOI: 10.11613/BM.2017.038
• EISSN: 1846-7482
• ISSN: 1330-0962

A nationwide multicentre study was conducted to establish well-defined reference intervals (RIs) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs).
K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. RIs were computed by the parametric method with/without applying the LAVE method.
Analyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those RIs were derived for each manufacturer. RIs were determined from all volunteers' results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific RIs were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific RIs were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC.
With the novel use of a freshly prepared blood panel, manufacturer-specific RIs' were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in RIs were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude.