Comparison of bioassays and laboratory assays for apple stem grooving virus

• Published in: Journal of virological methods Vol. 93; no. 1; pp. 167 - 173
• Main Authors: Kirby, M.J., Guise, C.M., Adams, A.N.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01-04-2001; Amsterdam Elsevier; New York, NY
• Subjects: Apple stem grooving virus; Base Sequence; Biological and medical sciences; ELISA; Field assays; Fruit - virology; Fundamental and applied biological sciences. Psychology; Genome, Viral; Glasshouse assays; IC-PCR; Indicators and Reagents; Malus micromalus; Microbiology; Molecular Sequence Data; Plant Diseases - virology; Plant Viruses - genetics; Plant Viruses - isolation & purification; Polymerase Chain Reaction; Sequence Alignment; Trees - virology; Virus Latency; Polymerase chain reaction; Apple stem grooving virus; IC-PCR; Field assays; Glasshouse assays; ELISA; Pyrus communis; Bioassay; Rosaceae; Virus; Capillovirus; Dicotyledones; Angiospermae; Spermatophyta; Detection; Malus; ELISA assay
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(01)00271-3

The standard field double-budding assay with the indicator Virginia Crab and the glasshouse test with the indicator
Malus micromalus, were compared with ELISA and immunocapture PCR for the detection of
Apple stem grooving virus (ASGV) in 102 apple trees and three oriental pear. Twenty-two trees were positive for ASGV by both ELISA and IC-PCR but three of these trees were negative by Virginia Crab, three were negative by
M. micromalus and one was negative by both these bioassays. The infected trees were re-tested by IC-PCR and ELISA in a second year; the IC-PCR results were confirmed but two of the 22 infected trees were negative by ELISA. On this evidence, IC-PCR is a more reliable assay for ASGV than the slow and expensive bioassays.