Properties of glutaminase of crayfish CNS: implications for axon–glia signaling

Glutaminase of crayfish axons is believed to participate in recycling of axon–glia signaling agent(s). We measured the activity and properties of glutaminase in crude homogenates of crayfish CNS, using ion exchange chromatography to separate radiolabeled product from substrate. Crayfish glutaminase...

Full description

Saved in:

Bibliographic Details
Published in:	Neuroscience Vol. 114; no. 3; pp. 699 - 705
Main Authors:	Engler, J.A, Gottesman, J.M, Harkins, J.C, Urazaev, A.K, Lieberman, E.M, Grossfeld, R.M
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-01-2002 Elsevier
Subjects:	Animals Astacidea Astacoidea - enzymology Axons - drug effects Axons - enzymology Biochemistry. Physiology. Immunology Biological and medical sciences Central Nervous System - drug effects Central Nervous System - enzymology Crustacea Fundamental and applied biological sciences. Psychology glutamate Glutaminase - antagonists & inhibitors Glutaminase - metabolism glutamine Invertebrates N-acetylaspartylglutamate Neuroglia - drug effects Neuroglia - enzymology Physiology. Development Signal Transduction - drug effects Signal Transduction - physiology N-acetylaspartylglutamate DON, 6-diazo-5-oxo- L-norleucine NCS, normal (physiological) saline PAG, phosphate-activated glutaminase GAD, glutamate decarboxylase PIG, phosphate-independent glutaminase glutamine glutamate NAAG, N-acetylaspartylglutamate Glial cell Enzyme Neuroglia Central nervous system Axon Glutamate Glutaminase Macrura Crustacea Arthropoda Excitatory aminoacid Neurotransmitter Hydrolases Decapoda Procambarus clarkii Invertebrata Glutamine
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Glutaminase of crayfish axons is believed to participate in recycling of axon–glia signaling agent(s). We measured the activity and properties of glutaminase in crude homogenates of crayfish CNS, using ion exchange chromatography to separate radiolabeled product from substrate. Crayfish glutaminase activity is cytoplasmic and/or weakly bound to membranes and dependent on time, tissue protein, and glutamine concentration. It resembles the kidney-type phosphate-activated glutaminase of mammals in being stimulated by inorganic phosphate and alkaline pH and inhibited by the product glutamate and by the glutamine analog 6-diazo-5-oxo- L-norleucine. During incubation of crayfish CNS fibers in Na +-free saline containing radiolabeled glutamine, there is an increased formation of radiolabeled glutamate in axoplasm that is temporally associated with an increase in axonal pH from about 7.1 to about 8.0. Both the formation of glutamate and the change in pH are reduced by 6-diazo-5-oxo- L-norleucine. Our results suggest that crayfish glutaminase activity is regulated by cellular changes in pH and glutamate concentration. Such changes could impact availability of the axon–glia signaling agents glutamate and N-acetylaspartylglutamate.
AbstractList	Glutaminase of crayfish axons is believed to participate in recycling of axon-glia signaling agent(s). We measured the activity and properties of glutaminase in crude homogenates of crayfish CNS, using ion exchange chromatography to separate radiolabeled product from substrate. Crayfish glutaminase activity is cytoplasmic and/or weakly bound to membranes and dependent on time, tissue protein, and glutamine concentration. It resembles the kidney-type phosphate-activated glutaminase of mammals in being stimulated by inorganic phosphate and alkaline pH and inhibited by the product glutamate and by the glutamine analog 6-diazo-5-oxo-L-norleucine. During incubation of crayfish CNS fibers in Na super(+)-free saline containing radiolabeled glutamine, there is an increased formation of radiolabeled glutamate in axoplasm that is temporally associated with an increase in axonal pH from about 7.1 to about 8.0. Both the formation of glutamate and the change in pH are reduced by 6-diazo-5-oxo-L-norleucine. Our results suggest that crayfish glutaminase activity is regulated by cellular changes in pH and glutamate concentration. Such changes could impact availability of the axon-glia signaling agents glutamate and N-acetylaspartyl-glutamate. Glutaminase of crayfish axons is believed to participate in recycling of axon–glia signaling agent(s). We measured the activity and properties of glutaminase in crude homogenates of crayfish CNS, using ion exchange chromatography to separate radiolabeled product from substrate. Crayfish glutaminase activity is cytoplasmic and/or weakly bound to membranes and dependent on time, tissue protein, and glutamine concentration. It resembles the kidney-type phosphate-activated glutaminase of mammals in being stimulated by inorganic phosphate and alkaline pH and inhibited by the product glutamate and by the glutamine analog 6-diazo-5-oxo- L-norleucine. During incubation of crayfish CNS fibers in Na +-free saline containing radiolabeled glutamine, there is an increased formation of radiolabeled glutamate in axoplasm that is temporally associated with an increase in axonal pH from about 7.1 to about 8.0. Both the formation of glutamate and the change in pH are reduced by 6-diazo-5-oxo- L-norleucine. Our results suggest that crayfish glutaminase activity is regulated by cellular changes in pH and glutamate concentration. Such changes could impact availability of the axon–glia signaling agents glutamate and N-acetylaspartylglutamate. Glutaminase of crayfish axons is believed to participate in recycling of axon-glia signaling agent(s). We measured the activity and properties of glutaminase in crude homogenates of crayfish CNS, using ion exchange chromatography to separate radiolabeled product from substrate. Crayfish glutaminase activity is cytoplasmic and/or weakly bound to membranes and dependent on time, tissue protein, and glutamine concentration. It resembles the kidney-type phosphate-activated glutaminase of mammals in being stimulated by inorganic phosphate and alkaline pH and inhibited by the product glutamate and by the glutamine analog 6-diazo-5-oxo-L-norleucine. During incubation of crayfish CNS fibers in Na(+)-free saline containing radiolabeled glutamine, there is an increased formation of radiolabeled glutamate in axoplasm that is temporally associated with an increase in axonal pH from about 7.1 to about 8.0. Both the formation of glutamate and the change in pH are reduced by 6-diazo-5-oxo-L-norleucine. Our results suggest that crayfish glutaminase activity is regulated by cellular changes in pH and glutamate concentration. Such changes could impact availability of the axon-glia signaling agents glutamate and N-acetylaspartylglutamate.
Author	Harkins, J.C Grossfeld, R.M Lieberman, E.M Engler, J.A Urazaev, A.K Gottesman, J.M
Author_xml	– sequence: 1 givenname: J.A surname: Engler fullname: Engler, J.A organization: Zoology Department and W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC 27695-7617, USA – sequence: 2 givenname: J.M surname: Gottesman fullname: Gottesman, J.M organization: Zoology Department and W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC 27695-7617, USA – sequence: 3 givenname: J.C surname: Harkins fullname: Harkins, J.C organization: Zoology Department and W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC 27695-7617, USA – sequence: 4 givenname: A.K surname: Urazaev fullname: Urazaev, A.K organization: Department of Physiology, East Carolina University Brody School of Medicine, Greenville, NC 27858-4354, USA – sequence: 5 givenname: E.M surname: Lieberman fullname: Lieberman, E.M organization: Department of Physiology, East Carolina University Brody School of Medicine, Greenville, NC 27858-4354, USA – sequence: 6 givenname: R.M surname: Grossfeld fullname: Grossfeld, R.M email: robert_grossfeld@ncsu.edu organization: Zoology Department and W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC 27695-7617, USA
BackLink	http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13925244$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/12220571$$D View this record in MEDLINE/PubMed
BookMark	eNqFkM1q3DAQgEVJaDZJH6HFl5bm4GYkWZLdSylL_iD0h7RnIcujrYotbSVvaW55h7xhn6R2dmmOGQaGgW9--A7JXogBCXlJ4R0FKk9vgIMsK8HYW2AnAFyokj8jC1orXipRVXtk8R85IIc5_4QpRMWfkwPKGAOh6IJ8_ZLiGtPoMRfRFat-M5rBB5Nxbm0yt87nH8Xy0837wg_r3lsz-hhy4WIqzJ8Y_t7dr3pviuxXwfQ-rI7JvjN9xhe7ekS-n599W16W158vrpYfr0tbMTaWTS2aRlDhbO2UdAJ4rVoBjLeWycpIAa1TVgJaaC3Frqk7XkNTW9VShpzzI_Jmu3ed4q8N5lEPPlvsexMwbrJWDFSjuHwSpLUUgks2gWIL2hRzTuj0OvnBpFtNQc_S9YN0PRvVMOUsXc-fvNod2LQDdo9TO8sT8HoHmGxN75IJ1udHjjdMsKqauA9bDidvvz0mna3HYLHzCe2ou-ifeOUfO-ufvA
CODEN	NRSCDN
CitedBy_id	crossref_primary_10_1016_j_bbrc_2013_11_043 crossref_primary_10_1172_JCI75836 crossref_primary_10_1016_j_ecoenv_2009_11_006 crossref_primary_10_3233_JAD_190588 crossref_primary_10_1007_s00359_011_0653_6 crossref_primary_10_1016_j_bbrc_2013_06_110 crossref_primary_10_1021_acschembio_6b00493 crossref_primary_10_3390_ijms160922830 crossref_primary_10_1002_jez_561 crossref_primary_10_1016_S1096_4959_02_00124_0 crossref_primary_10_1172_jci_insight_160345
Cites_doi	10.1016/0028-3908(86)90108-5 10.1016/0003-2697(83)90317-2 10.1042/bj2820559 10.1007/BF00232585 10.1016/0065-2571(94)00003-L 10.1042/bj3050837 10.1002/glia.440140305 10.1016/0003-2697(70)90069-2 10.1201/9781351072298-5 10.1007/BF00967289 10.1385/0-89603-316-3:129 10.1002/(SICI)1098-1136(199912)28:3<175::AID-GLIA1>3.0.CO;2-7 10.1016/S0306-4522(01)00270-6 10.1152/ajpcell.1994.267.6.C1633 10.1016/S0005-2736(96)00189-7 10.1007/BF00965528 10.1146/annurev.nu.15.070195.001025 10.1016/S0306-4522(00)00054-3 10.1111/j.1748-1716.1967.tb03640.x 10.1016/0006-8993(89)91533-3 10.1023/A:1007668801570 10.1093/jn/130.4.1026S 10.1016/0301-0082(94)90032-9 10.1016/S0306-4522(01)00393-1 10.1016/S0076-6879(85)13033-8 10.1074/jbc.272.17.11276 10.1016/S0306-4522(01)00271-8 10.1007/BF00585877 10.1073/pnas.54.3.778 10.1111/j.1471-4159.1971.tb00183.x 10.1016/0003-2697(85)90442-7
ContentType	Journal Article
Copyright	2002 IBRO 2003 INIST-CNRS Copyright 2002 IBRO
Copyright_xml	– notice: 2002 IBRO – notice: 2003 INIST-CNRS – notice: Copyright 2002 IBRO
DBID	IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION F1W H95 L.G 7X8
DOI	10.1016/S0306-4522(02)00357-3
DatabaseName	Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ASFA: Aquatic Sciences and Fisheries Abstracts Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources Aquatic Science & Fisheries Abstracts (ASFA) Professional MEDLINE - Academic
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources Aquatic Science & Fisheries Abstracts (ASFA) Professional ASFA: Aquatic Sciences and Fisheries Abstracts MEDLINE - Academic
DatabaseTitleList	Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources MEDLINE MEDLINE - Academic
Database_xml	– sequence: 1 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Anatomy & Physiology
EISSN	1873-7544
EndPage	705
ExternalDocumentID	10_1016_S0306_4522_02_00357_3 12220571 13925244 S0306452202003573
Genre	Research Support, U.S. Gov't, P.H.S Journal Article
GrantInformation_xml	– fundername: NINDS NIH HHS grantid: 1 R01 NS34799-01A1
GroupedDBID	--- --K --M -DZ -~X .1- .55 .FO .GJ .~1 0R~ 123 1B1 1P~ 1RT 1~. 1~5 29N 4.4 457 4G. 53G 5RE 5VS 7-5 71M 8P~ 9JM AABNK AACTN AADPK AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXLA AAXUO ABCQJ ABFNM ABFRF ABJNI ABLJU ABMAC ABTEW ABXDB ABYKQ ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD AEBSH AEFWE AEKER AENEX AEVXI AFCTW AFKWA AFRHN AFTJW AFXIZ AGHFR AGUBO AGWIK AGYEJ AHHHB AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV AJUYK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CS3 DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HMQ HVGLF HZ~ IHE J1W KOM L7B M2V M41 MO0 MOBAO N9A O-L O9- OAUVE OP~ OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SCC SDF SDG SDP SES SEW SNS SPCBC SSN SSZ T5K UNMZH WUQ X7M YYP Z5R ZGI ZXP ~G- 08R AAUGY ABPIF ABPTK IQODW AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION F1W H95 L.G 7X8
ID	FETCH-LOGICAL-c422t-98599515fc8f76f50387b5023bc264a650bf7c60ec0bc1ed98d38098c7b12e333
ISSN	0306-4522
IngestDate	Fri Oct 25 07:01:35 EDT 2024 Fri Oct 25 21:01:44 EDT 2024 Thu Sep 26 17:06:19 EDT 2024 Sat Sep 28 08:37:43 EDT 2024 Sun Oct 22 16:05:41 EDT 2023 Fri Feb 23 02:28:54 EST 2024
IsPeerReviewed	true
IsScholarly	true
Issue	3
Keywords	N-acetylaspartylglutamate DON, 6-diazo-5-oxo- L-norleucine NCS, normal (physiological) saline PAG, phosphate-activated glutaminase GAD, glutamate decarboxylase PIG, phosphate-independent glutaminase glutamine glutamate NAAG, N-acetylaspartylglutamate Glial cell Enzyme Neuroglia Central nervous system Axon Glutamate Glutaminase Macrura Crustacea Arthropoda Excitatory aminoacid Neurotransmitter Hydrolases Decapoda Procambarus clarkii Invertebrata Glutamine
Language	English
License	CC BY 4.0 Copyright 2002 IBRO
LinkModel	OpenURL
MergedId	FETCHMERGED-LOGICAL-c422t-98599515fc8f76f50387b5023bc264a650bf7c60ec0bc1ed98d38098c7b12e333
Notes	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
PMID	12220571
PQID	18655362
PQPubID	23462
PageCount	7
ParticipantIDs	proquest_miscellaneous_72079736 proquest_miscellaneous_18655362 crossref_primary_10_1016_S0306_4522_02_00357_3 pubmed_primary_12220571 pascalfrancis_primary_13925244 elsevier_sciencedirect_doi_10_1016_S0306_4522_02_00357_3
PublicationCentury	2000
PublicationDate	2002-01-01
PublicationDateYYYYMMDD	2002-01-01
PublicationDate_xml	– month: 01 year: 2002 text: 2002-01-01 day: 01
PublicationDecade	2000
PublicationPlace	Oxford
PublicationPlace_xml	– name: Oxford – name: United States
PublicationTitle	Neuroscience
PublicationTitleAlternate	Neuroscience
PublicationYear	2002
Publisher	Elsevier Ltd Elsevier
Publisher_xml	– name: Elsevier Ltd – name: Elsevier
References	Lieberman, Hargittai, Grossfeld (BIB21) 1994; 44 Kvamme, Ingeborg, Svenneby (BIB35) 1985; 113 Bradford, Ward, Foley (BIB2) 1989; 476 Deitmer, Rose, Munsch, Schmidt, Nett, Schneider, Lohr (BIB7) 1999; 28 Kvamme, E., Svenneby, G., Torgner, I.A., 1988. Glutaminases. In: Kvamme, E. (Ed.), Glutamine and Glutamate in Mammals, vol. I. CRC, Boca Raton, FL, pp. 53–67. Gafurov, Urazaev, Grossfeld, Lieberman (BIB8) 2001; 106 Lieberman, Pascarella (BIB20) 1983; 134 Grossfeld, R.M., Hargittai, P.T., Lieberman, E.M., 1995. Glutamate-mediated neuron-glia signaling in invertebrates and vertebrates. In: Vernadakis, A., Roots, B.I. (Eds.), Neuron-Glia Interrelationships During Phylogeny. II. Plasticity and Regeneration. Humana, Totowa, NJ, pp. 129–159. Nissim (BIB26) 1999; 277 Kravitz, Molinoff, Hall (BIB14) 1965; 54 Sherman, Mott (BIB28) 1986; 25 Nelson, Rumsey, Erecinska (BIB23) 1992; 282 Newcomb, Sun, Taylor, Curthoys, Giffard (BIB24) 1997; 272 McKinnon, Hargittai, Grossfeld, Lieberman (BIB22) 1995; 14 Kane, Buttram, Urazaev, Lieberman (BIB12) 2000; 97 Kovacevic, Day, Collett, Brosnan, Brosnan (BIB13) 1995; 305 Smith, Krohn, Hermanson, Mallia, Gartner, Provenzano, Fujimoto, Goeke, Olson, Klenk (BIB29) 1985; 150 Curthoys, Watford (BIB5) 1995; 15 Brookes, Turner (BIB3) 1994; 267 Kvamme, E., 1983. Glutaminase (PAG). In: Hertz, L., Kvamme, E., McGeer, E.G., Schousboe, A. (Eds.), Glutamine, Glutamate, and GABA in the Central Nervous System. Liss, New York, pp. 51–67. Urazaev, Grossfeld, Fletcher, Speno, Gafurov, Buttram, Lieberman (BIB31) 2001; 106 Aledo, de Pedro, Gomez-Fabre, Nunez de Castro, Marquez (BIB1) 1997; 1323 Brosnan, Ewart, Squires (BIB4) 1995; 33 Kvamme, Svenneby, Hertz, Schousboe (BIB17) 1982; 7 Urazaev, Buttram, Deen, Gafurov, Slusher, Grossfeld, Lieberman (BIB32) 2001; 107 Wallin (BIB34) 1967; 70 Daikhin, Yudkoff (BIB6) 2000; 130 Lieberman, Lane (BIB19) 1976; 366 Wallace, Dawson (BIB33) 1992; 17 Grossfeld, Shooter (BIB9) 1970; 18 Kvamme, Roberg, Torgner (BIB16) 2000; 25 Tate, Meister (BIB30) 1981; 39 Nicklas, W.J., 1983. Relative contributions of neurons and glia to metabolism of glutamate and GABA. In: Hertz, L., Kvamme, E., McGeer, E.G., Schousboe, A. (Eds.), Glutamine, Glutamate, and GABA in the Central Nervous System. Liss, New York, pp. 219–231. Prusiner, Milner (BIB27) 1970; 37 Huseby, N.-E., 1988. Glutaminases. In: Kvamme, E. (Ed.), Glutamine and Glutamate in Mammals, vol. I. CRC, Boca Raton, FL, pp. 153–163. Kvamme (10.1016/S0306-4522(02)00357-3_BIB17) 1982; 7 Urazaev (10.1016/S0306-4522(02)00357-3_BIB31) 2001; 106 Brosnan (10.1016/S0306-4522(02)00357-3_BIB4) 1995; 33 Sherman (10.1016/S0306-4522(02)00357-3_BIB28) 1986; 25 Kvamme (10.1016/S0306-4522(02)00357-3_BIB35) 1985; 113 Prusiner (10.1016/S0306-4522(02)00357-3_BIB27) 1970; 37 Lieberman (10.1016/S0306-4522(02)00357-3_BIB20) 1983; 134 Wallin (10.1016/S0306-4522(02)00357-3_BIB34) 1967; 70 Kvamme (10.1016/S0306-4522(02)00357-3_BIB16) 2000; 25 Kane (10.1016/S0306-4522(02)00357-3_BIB12) 2000; 97 Lieberman (10.1016/S0306-4522(02)00357-3_BIB21) 1994; 44 10.1016/S0306-4522(02)00357-3_BIB25 McKinnon (10.1016/S0306-4522(02)00357-3_BIB22) 1995; 14 Grossfeld (10.1016/S0306-4522(02)00357-3_BIB9) 1970; 18 10.1016/S0306-4522(02)00357-3_BIB18 Brookes (10.1016/S0306-4522(02)00357-3_BIB3) 1994; 267 Smith (10.1016/S0306-4522(02)00357-3_BIB29) 1985; 150 Deitmer (10.1016/S0306-4522(02)00357-3_BIB7) 1999; 28 Lieberman (10.1016/S0306-4522(02)00357-3_BIB19) 1976; 366 Nelson (10.1016/S0306-4522(02)00357-3_BIB23) 1992; 282 Wallace (10.1016/S0306-4522(02)00357-3_BIB33) 1992; 17 Bradford (10.1016/S0306-4522(02)00357-3_BIB2) 1989; 476 Kravitz (10.1016/S0306-4522(02)00357-3_BIB14) 1965; 54 Curthoys (10.1016/S0306-4522(02)00357-3_BIB5) 1995; 15 Tate (10.1016/S0306-4522(02)00357-3_BIB30) 1981; 39 Daikhin (10.1016/S0306-4522(02)00357-3_BIB6) 2000; 130 Gafurov (10.1016/S0306-4522(02)00357-3_BIB8) 2001; 106 Urazaev (10.1016/S0306-4522(02)00357-3_BIB32) 2001; 107 10.1016/S0306-4522(02)00357-3_BIB10 Aledo (10.1016/S0306-4522(02)00357-3_BIB1) 1997; 1323 10.1016/S0306-4522(02)00357-3_BIB11 Newcomb (10.1016/S0306-4522(02)00357-3_BIB24) 1997; 272 Kovacevic (10.1016/S0306-4522(02)00357-3_BIB13) 1995; 305 Nissim (10.1016/S0306-4522(02)00357-3_BIB26) 1999; 277 10.1016/S0306-4522(02)00357-3_BIB15
References_xml	– volume: 106 start-page: 227 year: 2001 end-page: 235 ident: BIB8 article-title: publication-title: Neuroscience contributor: fullname: Lieberman – volume: 272 start-page: 11276 year: 1997 end-page: 11282 ident: BIB24 article-title: Increased production of extracellular glutamate by the mitochondrial glutaminase following neuronal death publication-title: J. Biol. Chem. contributor: fullname: Giffard – volume: 366 start-page: 189 year: 1976 end-page: 193 ident: BIB19 article-title: The influence of cardioactive steroids, metabolic inhibitors, temperature and sodium on membrane conductance and potential of crayfish giant axons publication-title: Pflugers Arch. contributor: fullname: Lane – volume: 282 start-page: 559 year: 1992 end-page: 564 ident: BIB23 article-title: Glutamine catabolism by heart muscle: Properties of phosphate-activated glutaminase publication-title: Biochem. J. contributor: fullname: Erecinska – volume: 25 start-page: 1353 year: 1986 end-page: 1357 ident: BIB28 article-title: Effects of glutaminase inhibition on release of endogenous glutamic acid publication-title: Neuropharmacology contributor: fullname: Mott – volume: 150 start-page: 76 year: 1985 end-page: 85 ident: BIB29 article-title: Measurement of protein using bicinchoninic acid publication-title: Anal. Biochem. contributor: fullname: Klenk – volume: 106 start-page: 237 year: 2001 end-page: 247 ident: BIB31 article-title: Synthesis and release of publication-title: Neuroscience contributor: fullname: Lieberman – volume: 134 start-page: 413 year: 1983 end-page: 423 ident: BIB20 article-title: Enzymatic determination of subpicomole quantities of phospho-L-arginine in nerve cytoplasm publication-title: Anal. Biochem. contributor: fullname: Pascarella – volume: 54 start-page: 778 year: 1965 end-page: 782 ident: BIB14 article-title: A comparison of the enzymes and substrates of gamma-aminobutyric acid metabolism in lobster excitatory and inhibitory axons publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Hall – volume: 113 start-page: 241 year: 1985 end-page: 263 ident: BIB35 article-title: Glutaminase from mammalian tissues publication-title: Methods Enzymol. contributor: fullname: Svenneby – volume: 14 start-page: 198 year: 1995 end-page: 208 ident: BIB22 article-title: Glutamine cycle enzymes in the crayfish giant nerve fiber: Implications for axon-to-glia signaling publication-title: Glia contributor: fullname: Lieberman – volume: 107 start-page: 697 year: 2001 end-page: 703 ident: BIB32 article-title: Mechanisms for clearance of released publication-title: Neuroscience contributor: fullname: Lieberman – volume: 70 start-page: 419 year: 1967 end-page: 430 ident: BIB34 article-title: Intracellular ion concentrations in single crayfish axons publication-title: Acta Physiol. Scand. contributor: fullname: Wallin – volume: 18 start-page: 2265 year: 1970 end-page: 2277 ident: BIB9 article-title: A study of the changes in protein composition of mouse brain during ontogenetic development publication-title: J. Neurochem. contributor: fullname: Shooter – volume: 17 start-page: 1113 year: 1992 end-page: 1122 ident: BIB33 article-title: Ammonia regulation of phosphate-activated glutaminase displays regional variation and impairment in the brain of aged rats publication-title: Neurochem. Res. contributor: fullname: Dawson – volume: 33 start-page: 131 year: 1995 end-page: 146 ident: BIB4 article-title: Hormonal control of hepatic glutaminase publication-title: Adv. Enzyme Regul. contributor: fullname: Squires – volume: 267 start-page: C1633 year: 1994 end-page: C1640 ident: BIB3 article-title: K publication-title: Am. J. Physiol. contributor: fullname: Turner – volume: 130 start-page: 1026S year: 2000 end-page: 1031S ident: BIB6 article-title: Compartmentation of brain glutamate metabolism in neurons and glia publication-title: J. Nutr. contributor: fullname: Yudkoff – volume: 7 start-page: 761 year: 1982 end-page: 770 ident: BIB17 article-title: Properties of phosphate-activated glutaminase in astrocytes cultured from mouse brain publication-title: Neurochem. Res. contributor: fullname: Schousboe – volume: 97 start-page: 601 year: 2000 end-page: 609 ident: BIB12 article-title: Uptake and metabolism of glutamate at non-synaptic regions of crayfish central nerve fibers: Implications for axon-glia signaling publication-title: Neuroscience contributor: fullname: Lieberman – volume: 44 start-page: 333 year: 1994 end-page: 376 ident: BIB21 article-title: Electrophysiological and metabolic interactions between axons and glia of crayfish and squid publication-title: Prog. Neurobiol. contributor: fullname: Grossfeld – volume: 28 start-page: 175 year: 1999 end-page: 182 ident: BIB7 article-title: Leech giant glial cell: Functional role in a simple nervous system publication-title: Glia contributor: fullname: Lohr – volume: 277 start-page: F493 year: 1999 end-page: F497 ident: BIB26 article-title: Newer aspects of glutamine/glutamate metabolism: The role of acute pH changes publication-title: Am. J. Physiol. contributor: fullname: Nissim – volume: 15 start-page: 133 year: 1995 end-page: 159 ident: BIB5 article-title: Regulation of glutaminase activity and glutamine metabolism publication-title: Annu. Rev. Nutr. contributor: fullname: Watford – volume: 37 start-page: 429 year: 1970 end-page: 438 ident: BIB27 article-title: A rapid radioactive assay for glutamine synthetase, glutaminase, asparagine synthetase, and asparaginase publication-title: Anal. Biochem. contributor: fullname: Milner – volume: 476 start-page: 29 year: 1989 end-page: 34 ident: BIB2 article-title: Glutaminase inhibition and the release of neurotransmitter glutamate from synaptosomes publication-title: Brain Res. contributor: fullname: Foley – volume: 25 start-page: 1407 year: 2000 end-page: 1419 ident: BIB16 article-title: Phosphate-activated glutaminase and mitochondrial glutamine transport in the brain publication-title: Neurochem. Res. contributor: fullname: Torgner – volume: 39 start-page: 357 year: 1981 end-page: 368 ident: BIB30 article-title: γ-Glutamyl transpeptidase: Catalytic, structural and functional aspects publication-title: Mol. Cell. Biochem. contributor: fullname: Meister – volume: 305 start-page: 837 year: 1995 end-page: 841 ident: BIB13 article-title: Activation of hepatic glutaminase by spermine publication-title: Biochem. J. contributor: fullname: Brosnan – volume: 1323 start-page: 173 year: 1997 end-page: 184 ident: BIB1 article-title: Submitochondrial localization and membrane topography of Ehrlich ascitic tumour cell glutaminase publication-title: Biochim. Biophys. Acta contributor: fullname: Marquez – volume: 25 start-page: 1353 year: 1986 ident: 10.1016/S0306-4522(02)00357-3_BIB28 article-title: Effects of glutaminase inhibition on release of endogenous glutamic acid publication-title: Neuropharmacology doi: 10.1016/0028-3908(86)90108-5 contributor: fullname: Sherman – volume: 134 start-page: 413 year: 1983 ident: 10.1016/S0306-4522(02)00357-3_BIB20 article-title: Enzymatic determination of subpicomole quantities of phospho-L-arginine in nerve cytoplasm publication-title: Anal. Biochem. doi: 10.1016/0003-2697(83)90317-2 contributor: fullname: Lieberman – volume: 282 start-page: 559 year: 1992 ident: 10.1016/S0306-4522(02)00357-3_BIB23 article-title: Glutamine catabolism by heart muscle: Properties of phosphate-activated glutaminase publication-title: Biochem. J. doi: 10.1042/bj2820559 contributor: fullname: Nelson – volume: 39 start-page: 357 year: 1981 ident: 10.1016/S0306-4522(02)00357-3_BIB30 article-title: γ-Glutamyl transpeptidase: Catalytic, structural and functional aspects publication-title: Mol. Cell. Biochem. doi: 10.1007/BF00232585 contributor: fullname: Tate – volume: 33 start-page: 131 year: 1995 ident: 10.1016/S0306-4522(02)00357-3_BIB4 article-title: Hormonal control of hepatic glutaminase publication-title: Adv. Enzyme Regul. doi: 10.1016/0065-2571(94)00003-L contributor: fullname: Brosnan – volume: 305 start-page: 837 year: 1995 ident: 10.1016/S0306-4522(02)00357-3_BIB13 article-title: Activation of hepatic glutaminase by spermine publication-title: Biochem. J. doi: 10.1042/bj3050837 contributor: fullname: Kovacevic – volume: 14 start-page: 198 year: 1995 ident: 10.1016/S0306-4522(02)00357-3_BIB22 article-title: Glutamine cycle enzymes in the crayfish giant nerve fiber: Implications for axon-to-glia signaling publication-title: Glia doi: 10.1002/glia.440140305 contributor: fullname: McKinnon – volume: 37 start-page: 429 year: 1970 ident: 10.1016/S0306-4522(02)00357-3_BIB27 article-title: A rapid radioactive assay for glutamine synthetase, glutaminase, asparagine synthetase, and asparaginase publication-title: Anal. Biochem. doi: 10.1016/0003-2697(70)90069-2 contributor: fullname: Prusiner – ident: 10.1016/S0306-4522(02)00357-3_BIB18 doi: 10.1201/9781351072298-5 – volume: 17 start-page: 1113 year: 1992 ident: 10.1016/S0306-4522(02)00357-3_BIB33 article-title: Ammonia regulation of phosphate-activated glutaminase displays regional variation and impairment in the brain of aged rats publication-title: Neurochem. Res. doi: 10.1007/BF00967289 contributor: fullname: Wallace – ident: 10.1016/S0306-4522(02)00357-3_BIB10 doi: 10.1385/0-89603-316-3:129 – volume: 28 start-page: 175 year: 1999 ident: 10.1016/S0306-4522(02)00357-3_BIB7 article-title: Leech giant glial cell: Functional role in a simple nervous system publication-title: Glia doi: 10.1002/(SICI)1098-1136(199912)28:3<175::AID-GLIA1>3.0.CO;2-7 contributor: fullname: Deitmer – volume: 106 start-page: 237 year: 2001 ident: 10.1016/S0306-4522(02)00357-3_BIB31 article-title: Synthesis and release of N-acetylaspartylglutamate (NAAG) by crayfish nerve fibers: Implications for axon–glia signaling publication-title: Neuroscience doi: 10.1016/S0306-4522(01)00270-6 contributor: fullname: Urazaev – volume: 267 start-page: C1633 year: 1994 ident: 10.1016/S0306-4522(02)00357-3_BIB3 article-title: K+-induced alkalinization in mouse cerebral astrocytes mediated by reversal of electrogenic Na+-HCO3− cotransport publication-title: Am. J. Physiol. doi: 10.1152/ajpcell.1994.267.6.C1633 contributor: fullname: Brookes – volume: 1323 start-page: 173 year: 1997 ident: 10.1016/S0306-4522(02)00357-3_BIB1 article-title: Submitochondrial localization and membrane topography of Ehrlich ascitic tumour cell glutaminase publication-title: Biochim. Biophys. Acta doi: 10.1016/S0005-2736(96)00189-7 contributor: fullname: Aledo – volume: 7 start-page: 761 year: 1982 ident: 10.1016/S0306-4522(02)00357-3_BIB17 article-title: Properties of phosphate-activated glutaminase in astrocytes cultured from mouse brain publication-title: Neurochem. Res. doi: 10.1007/BF00965528 contributor: fullname: Kvamme – ident: 10.1016/S0306-4522(02)00357-3_BIB11 – volume: 277 start-page: F493 issue: Renal Physiol. 46 year: 1999 ident: 10.1016/S0306-4522(02)00357-3_BIB26 article-title: Newer aspects of glutamine/glutamate metabolism: The role of acute pH changes publication-title: Am. J. Physiol. contributor: fullname: Nissim – volume: 15 start-page: 133 year: 1995 ident: 10.1016/S0306-4522(02)00357-3_BIB5 article-title: Regulation of glutaminase activity and glutamine metabolism publication-title: Annu. Rev. Nutr. doi: 10.1146/annurev.nu.15.070195.001025 contributor: fullname: Curthoys – volume: 97 start-page: 601 year: 2000 ident: 10.1016/S0306-4522(02)00357-3_BIB12 article-title: Uptake and metabolism of glutamate at non-synaptic regions of crayfish central nerve fibers: Implications for axon-glia signaling publication-title: Neuroscience doi: 10.1016/S0306-4522(00)00054-3 contributor: fullname: Kane – volume: 70 start-page: 419 year: 1967 ident: 10.1016/S0306-4522(02)00357-3_BIB34 article-title: Intracellular ion concentrations in single crayfish axons publication-title: Acta Physiol. Scand. doi: 10.1111/j.1748-1716.1967.tb03640.x contributor: fullname: Wallin – volume: 476 start-page: 29 year: 1989 ident: 10.1016/S0306-4522(02)00357-3_BIB2 article-title: Glutaminase inhibition and the release of neurotransmitter glutamate from synaptosomes publication-title: Brain Res. doi: 10.1016/0006-8993(89)91533-3 contributor: fullname: Bradford – ident: 10.1016/S0306-4522(02)00357-3_BIB15 – volume: 25 start-page: 1407 year: 2000 ident: 10.1016/S0306-4522(02)00357-3_BIB16 article-title: Phosphate-activated glutaminase and mitochondrial glutamine transport in the brain publication-title: Neurochem. Res. doi: 10.1023/A:1007668801570 contributor: fullname: Kvamme – ident: 10.1016/S0306-4522(02)00357-3_BIB25 – volume: 130 start-page: 1026S year: 2000 ident: 10.1016/S0306-4522(02)00357-3_BIB6 article-title: Compartmentation of brain glutamate metabolism in neurons and glia publication-title: J. Nutr. doi: 10.1093/jn/130.4.1026S contributor: fullname: Daikhin – volume: 44 start-page: 333 year: 1994 ident: 10.1016/S0306-4522(02)00357-3_BIB21 article-title: Electrophysiological and metabolic interactions between axons and glia of crayfish and squid publication-title: Prog. Neurobiol. doi: 10.1016/0301-0082(94)90032-9 contributor: fullname: Lieberman – volume: 107 start-page: 697 year: 2001 ident: 10.1016/S0306-4522(02)00357-3_BIB32 article-title: Mechanisms for clearance of released N-acetylaspartylglutamate in crayfish nerve fibers: Implications for axon-glia signaling publication-title: Neuroscience doi: 10.1016/S0306-4522(01)00393-1 contributor: fullname: Urazaev – volume: 113 start-page: 241 year: 1985 ident: 10.1016/S0306-4522(02)00357-3_BIB35 article-title: Glutaminase from mammalian tissues publication-title: Methods Enzymol. doi: 10.1016/S0076-6879(85)13033-8 contributor: fullname: Kvamme – volume: 272 start-page: 11276 year: 1997 ident: 10.1016/S0306-4522(02)00357-3_BIB24 article-title: Increased production of extracellular glutamate by the mitochondrial glutaminase following neuronal death publication-title: J. Biol. Chem. doi: 10.1074/jbc.272.17.11276 contributor: fullname: Newcomb – volume: 106 start-page: 227 year: 2001 ident: 10.1016/S0306-4522(02)00357-3_BIB8 article-title: N-Acetylaspartylglutamate (NAAG) is the probable mediator of axon-to-glia signaling in the crayfish medial giant nerve fiber publication-title: Neuroscience doi: 10.1016/S0306-4522(01)00271-8 contributor: fullname: Gafurov – volume: 366 start-page: 189 year: 1976 ident: 10.1016/S0306-4522(02)00357-3_BIB19 article-title: The influence of cardioactive steroids, metabolic inhibitors, temperature and sodium on membrane conductance and potential of crayfish giant axons publication-title: Pflugers Arch. doi: 10.1007/BF00585877 contributor: fullname: Lieberman – volume: 54 start-page: 778 year: 1965 ident: 10.1016/S0306-4522(02)00357-3_BIB14 article-title: A comparison of the enzymes and substrates of gamma-aminobutyric acid metabolism in lobster excitatory and inhibitory axons publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.54.3.778 contributor: fullname: Kravitz – volume: 18 start-page: 2265 year: 1970 ident: 10.1016/S0306-4522(02)00357-3_BIB9 article-title: A study of the changes in protein composition of mouse brain during ontogenetic development publication-title: J. Neurochem. doi: 10.1111/j.1471-4159.1971.tb00183.x contributor: fullname: Grossfeld – volume: 150 start-page: 76 year: 1985 ident: 10.1016/S0306-4522(02)00357-3_BIB29 article-title: Measurement of protein using bicinchoninic acid publication-title: Anal. Biochem. doi: 10.1016/0003-2697(85)90442-7 contributor: fullname: Smith
SSID	ssj0000543
Score	1.7695978
Snippet	Glutaminase of crayfish axons is believed to participate in recycling of axon–glia signaling agent(s). We measured the activity and properties of glutaminase... Glutaminase of crayfish axons is believed to participate in recycling of axon-glia signaling agent(s). We measured the activity and properties of glutaminase...
SourceID	proquest crossref pubmed pascalfrancis elsevier
SourceType	Aggregation Database Index Database Publisher
StartPage	699
SubjectTerms	Animals Astacidea Astacoidea - enzymology Axons - drug effects Axons - enzymology Biochemistry. Physiology. Immunology Biological and medical sciences Central Nervous System - drug effects Central Nervous System - enzymology Crustacea Fundamental and applied biological sciences. Psychology glutamate Glutaminase - antagonists & inhibitors Glutaminase - metabolism glutamine Invertebrates N-acetylaspartylglutamate Neuroglia - drug effects Neuroglia - enzymology Physiology. Development Signal Transduction - drug effects Signal Transduction - physiology
Title	Properties of glutaminase of crayfish CNS: implications for axon–glia signaling
URI	https://dx.doi.org/10.1016/S0306-4522(02)00357-3 https://www.ncbi.nlm.nih.gov/pubmed/12220571 https://search.proquest.com/docview/18655362 https://search.proquest.com/docview/72079736
Volume	114
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwELe67gUJIWB8dMDwA0IglOLESZzwVo3CKNIE6ibtzbKdZBrakqkfEuOJ_4H_kL-EuziJW1DFh8RL1FpunPp-vvv5cncm5ElstGYRY7CQmPJCHYWexj2Pgt1zmKpIKYbZyAdTcXiSvB6H416vPQHMtf1XSUMbyBozZ_9C2t1NoQE-g8zhClKH6x_J_QN612dYJhV54CkMpTDaxbrszUxdFVjDaP9wir6As9V48jqe8jNQwSb-gZ-en6kXGOChzlsD96kt9tRVweyAgQnBVv6TYQeLt9UCuGzjZZ0MnetVoY_eYmjYOWqPZ-qLgj-M-mr4fs0hEfzkkOgyZVxYUp2dxWIPq7dbu2OVbSK4hwX41rSxzSltYMdXdGtsT1JqzLSos7V_tQDWGTHtxgOejjVm0WEbgTp1Zq8LRqz7YldWV4cVfItsB6C2wj7ZHr0bn0ycZY9sFGZ7b5cR9tIN-IwFz5vBNnGd65dqDiuwsEenbN7b1Bzn6Ca50WxO6Mii6hbp5eVtsjMq1aK6uKJPaR0uXL-H2SEfHdBoVdAVoOHXFmgUgPaKrsKMAswowuz7128IMNoB7A45fjM-2j_wmvM5PBMGwcJLE6xW52MUYCHiAgsLCR0BCdQGaLYC7q8LYWKWG6aNn2dpkvGEpYkR2g9yzvld0i-rMr9PaBZoPzM6zDTWhwOOFGWJYSbxDUxlxs2ADNuZlJe2DIt08Ykw9RKnXrJA1lMv-YAk7XzLZjlYjigBKL_76d6afNyAsJeIgA4PyONWYBKUMb5hU2VeLefSxzRvoISbe4iAiVTweEDuWUm7uweY8y783X9_8gfkmluQD0l_MVvmj8jWPFvuNVD-ARjbuBw
link.rule.ids	315,782,786,27934,27935
linkProvider	Elsevier
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Properties+of+glutaminase+of+crayfish+CNS%3A+implications+for+axon%E2%80%93glia+signaling&rft.jtitle=Neuroscience&rft.au=Engler%2C+J.A&rft.au=Gottesman%2C+J.M&rft.au=Harkins%2C+J.C&rft.au=Urazaev%2C+A.K&rft.date=2002-01-01&rft.pub=Elsevier+Ltd&rft.issn=0306-4522&rft.eissn=1873-7544&rft.volume=114&rft.issue=3&rft.spage=699&rft.epage=705&rft_id=info:doi/10.1016%2FS0306-4522%2802%2900357-3&rft.externalDocID=S0306452202003573
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0306-4522&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0306-4522&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0306-4522&client=summon