Multicenter, prospective, observational study for urinary exosomal biomarkers of kidney allograft fibrosis

• Published in: Scientific reports Vol. 14; no. 1; pp. 20319 - 12
• Main Authors: Kim, Mi Joung, Kwon, Hye Eun, Jang, Hye-Won, Kim, Jin-Myung, Lee, Jae Jun, Jung, Joo Hee, Ko, Youngmin, Kwon, Hyunwook, Kim, Young Hoon, Jun, Heungman, Park, Sang Jun, Gwon, Jun Gyo, Shin, Sung
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02-09-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 692/53; 692/53/2421; Adult; Allografts; Atrophy; Biomarkers; Biomarkers - urine; Biopsy; Exosomes; Exosomes - metabolism; Female; Fibrosis; Glomerular Filtration Rate; Humanities and Social Sciences; Humans; Kidney - pathology; Kidney transplantation; Kidney Transplantation - adverse effects; Kidneys; Male; MicroRNAs; MicroRNAs - genetics; MicroRNAs - urine; Middle Aged; miRNA; multidisciplinary; Observational studies; Prospective Studies; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-71279-5

Severity of deceased donor kidney fibrosis impacts graft survival in deceased-donor kidney transplantation. Our aim was to identify potential miRNA biomarkers in urinary exosomes that mirror interstitial fibrosis and tubular atrophy (IFTA) severity. Among 109 urine samples from deceased donors, 34 displayed no IFTA in the zero-day biopsy (No IFTA group), while the remaining 75 deceased donor kidneys exhibited an IFTA score ≥ 1 (IFTA group). After analyzing previous reports and electronic databases, six miRNAs (miR-19, miR-21, miR-29c, miR-150, miR-200b, and miR-205) were selected as potential IFTA biomarker candidates. MiR-21, miR-29c, miR-150, and miR-205 levels were significantly higher, while miR-19 expression was significantly lower in the IFTA group. MiR-21 (AUC = 0.762; P < 0.001) and miR-29c (AUC = 0.795; P < 0.001) showed good predictive accuracy for IFTA. In the No IFTA group, the eGFR level at 1 week after transplantation was significantly higher compared to the IFTA group (41.34 mL/min/1.73m
2
vs. 28.65 mL/min/1.73m
2
, P = 0.012). These findings signify the potential of urinary exosomal miRNAs as valuable biomarker candidates for evaluating the severity of IFTA in deceased donor kidneys before they undergo recovery.