Mesoporous SBA-15 HPLC evaluation for controlled gentamicin drug delivery

• Published in: Journal of controlled release Vol. 97; no. 1; pp. 125 - 132
• Main Authors: Doadrio, A.L., Sousa, E.M.B., Doadrio, J.C., Pérez Pariente, J., Izquierdo-Barba, I., Vallet-Regı́, M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 31-05-2004; Elsevier
• Subjects: Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Delayed-Action Preparations - administration & dosage; Delayed-Action Preparations - analysis; Delayed-Action Preparations - chemistry; Drug Delivery Systems - methods; Drug Evaluation, Preclinical - methods; General pharmacology; Gentamicin; Gentamicins - administration & dosage; Gentamicins - analysis; Gentamicins - chemistry; HPLC; Medical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; SBA-15; Technology, Pharmaceutical - methods; SBA-15; Gentamicin; HPLC; Drug; Evaluation; Antibiotic; Aminoglycoside; HPLC chromatography; Antibacterial agent
• ISSN: 0168-3659; 1873-4995
• DOI: 10.1016/j.jconrel.2004.03.005

Mesoporous silica SBA-15 was prepared to evaluate its application as gentamicin drug delivery system. Two procedures were used to evaluate the delivery: calcined powder and disk conformed. The samples were charged with gentamicin sulphate and the experiments were carried out in vitro. No significant difference between powder and disk was observed in the tests. The release profiles exhibited a pronounced initial burst release effect of 60%, followed by a very slow release pattern. A new HPLC method was employed for calculated gentamicin amount in the delivery test. This method requires a small amount of sample, very advisable in these kinds of assays.