Direct determination of verapamil in urine and serum samples by micellar liquid chromatography and fluorescence detection
Verapamil, a calcium channel antagonist, is one of the most commonly prescribed drugs in the treatment of hypertension. In this work, it was determined in serum and urine samples by a sensitive and precise chromatographic procedure without any pre-treatment step in a C18 column using a micellar mobi...
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| Published in: | Journal of chromatography. B Vol. 839; no. 1; pp. 89 - 94 |
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| Main Authors: | , , , , |
| Format: | Journal Article Conference Proceeding |
| Language: | English |
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24-07-2006
Elsevier Science |
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| Abstract | Verapamil, a calcium channel antagonist, is one of the most commonly prescribed drugs in the treatment of hypertension. In this work, it was determined in serum and urine samples by a sensitive and precise chromatographic procedure without any pre-treatment step in a C18 column using a micellar mobile phase of 0.15
M sodium dodecyl sulfate and 5% pentanol at pH 7. Fluorescence detection set at 230
nm (excitation) and 312
nm (emission) was used. Verapamil is eluted at 12.5
min with no interference by the protein band or endogenous compounds. Linearities (
r
>
0.998), as well as intra- and inter-day precision, were studied in the validation of the method. LODs were also calculated to be 11.0, 18.5 and 20.2
ng/mL in micellar solution, serum and urine, respectively. Recoveries in the biological matrices were in the 97–99% range. Drug excretion in urine was studied in a volunteer receiving treatment for hypertension, and verapamil, as an unchanged drug, was separated from other metabolites. The procedure developed can be useful in the field of toxicology and clinical analysis. |
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| AbstractList | Verapamil, a calcium channel antagonist, is one of the most commonly prescribed drugs in the treatment of hypertension. In this work, it was determined in serum and urine samples by a sensitive and precise chromatographic procedure without any pre-treatment step in a C18 column using a micellar mobile phase of 0.15
M sodium dodecyl sulfate and 5% pentanol at pH 7. Fluorescence detection set at 230
nm (excitation) and 312
nm (emission) was used. Verapamil is eluted at 12.5
min with no interference by the protein band or endogenous compounds. Linearities (
r
>
0.998), as well as intra- and inter-day precision, were studied in the validation of the method. LODs were also calculated to be 11.0, 18.5 and 20.2
ng/mL in micellar solution, serum and urine, respectively. Recoveries in the biological matrices were in the 97–99% range. Drug excretion in urine was studied in a volunteer receiving treatment for hypertension, and verapamil, as an unchanged drug, was separated from other metabolites. The procedure developed can be useful in the field of toxicology and clinical analysis. Verapamil, a calcium channel antagonist, is one of the most commonly prescribed drugs in the treatment of hypertension. In this work, it was determined in serum and urine samples by a sensitive and precise chromatographic procedure without any pre-treatment step in a C18 column using a micellar mobile phase of 0.15M sodium dodecyl sulfate and 5% pentanol at pH 7. Fluorescence detection set at 230 nm (excitation) and 312 nm (emission) was used. Verapamil is eluted at 12.5 min with no interference by the protein band or endogenous compounds. Linearities (r > 0.998), as well as intra- and inter-day precision, were studied in the validation of the method. LODs were also calculated to be 11.0, 18.5 and 20.2 ng/mL in micellar solution, serum and urine, respectively. Recoveries in the biological matrices were in the 97-99% range. Drug excretion in urine was studied in a volunteer receiving treatment for hypertension, and verapamil, as an unchanged drug, was separated from other metabolites. The procedure developed can be useful in the field of toxicology and clinical analysis. |
| Author | Capella-Peiró, M.E. Esteve-Romero, J.S. Rambla-Alegre, M. Gil-Agustí, M.T. Carda-Broch, S. |
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| Cites_doi | 10.2165/00003088-199834060-00003 10.1016/j.trac.2004.11.003 10.1016/S0731-7085(00)00585-9 10.1016/S0379-0738(03)00056-2 10.1016/j.ejps.2004.04.006 10.1016/S0378-5173(02)00069-8 10.1016/j.forsciint.2004.02.017 10.1016/j.jpba.2004.11.003 10.1016/S0939-6411(01)00189-8 10.1021/ac970223g 10.1016/S0378-4347(99)00307-2 10.1016/S0003-2697(02)00309-3 10.1016/S0731-7085(02)00267-4 10.1016/0167-5273(81)90056-5 10.1002/j.1552-4604.1996.tb04148.x 10.1016/0002-9343(93)90314-F 10.1172/JCI112505 10.1007/BF02497477 |
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| Keywords | Urine Micellar mobile phase Verapamil Serum Direct injection Biological fluid Fluorescence detector Calcium antagonist Aralkylamine Liquid chromatography Antiarrhythmic agent Quantitative analysis |
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| SubjectTerms | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Calibration Chromatography, Micellar Electrokinetic Capillary Direct injection Fluorescence Fundamental and applied biological sciences. Psychology General pharmacology Humans Medical sciences Micellar mobile phase Pharmacology. Drug treatments Reproducibility of Results Serum Urine Verapamil Verapamil - blood Verapamil - chemistry Verapamil - urine |
| Title | Direct determination of verapamil in urine and serum samples by micellar liquid chromatography and fluorescence detection |
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