Application of short monolithic columns for improved detection of viruses

• Published in: Journal of virological methods Vol. 110; no. 2; pp. 163 - 171
• Main Authors: Branovic, Karmen, Forcic, Dubravko, Ivancic, Jelena, Strancar, Ales, Barut, Milos, Kosutic-Gulija, Tanja, Zgorelec, Renata, Mazuran, Renata
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 30-06-2003; Amsterdam Elsevier; New York, NY
• Subjects: Biological and medical sciences; Chromatography, DEAE-Cellulose - instrumentation; Chromatography, DEAE-Cellulose - methods; Chromatography, High Pressure Liquid; Concentration of viral RNA; Fundamental and applied biological sciences. Psychology; Humans; Measles Vaccine; Measles virus - genetics; Measles virus - isolation & purification; Microbiology; Monolithic columns; Mumps Vaccine; Mumps virus - genetics; Mumps virus - isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - isolation & purification; Techniques used in virology; Vaccines, Attenuated; Virology; Viruses; Concentration of viral RNA; Viruses; Monolithic columns; RNA; Method; Chromatography; Paramyxoviridae; Virus; Paramyxovirinae; Mononegavirales; Rubulavirus; Morbillivirus; Models; Detection; Mumps virus; Measles virus
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(03)00125-3

Monolithic chromatography media represent a novel generation of stationary phases introduced in the last 10–15 years providing a chromatography matrix with enhanced mass transfer and hydrodynamic properties. These features allow for an efficient and fast separation of especially large biomolecules like e.g., DNA and viruses. In this study, the enrichment of virus RNA on short monolithic columns prior to molecular detection of viruses is described. Measles and mumps viruses were chosen as model viruses. The results show that it is possible to bind viral RNA on monoliths and concentrate viral nucleic acids from a fairly dilute sample. Consequently, a potential application of short monolithic columns is the concentration of virus RNA to improve the sensitivity and selectivity of viral detection with the possibility of isolating viral RNA from cell-free biological fluids.