Immunoglobulin production by cultured human lymphocytes

• Published in: Journal of Immunological Methods Vol. 238; no. 1; pp. 3 - 15
• Main Author: Ling, Noel R
• Format: Book Review Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 21-04-2000; Elsevier
• Subjects: Animals; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Biological and medical sciences; Biomarkers; Biotechnology; Cell Differentiation; Cell Division; Cells, Cultured; Culture; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; Humans; Immunoglobulin; Immunoglobulin Class Switching; Immunoglobulin Variable Region; Immunoglobulins - biosynthesis; Industrial applications and implications. Economical aspects; Lymphocyte Activation; Lymphocytes; Monoclonal antibodies; Production of active biomolecules; TNF, tumour necrosis factor; Ab, antibody; mAb, monoclonal antibody; GC, germinal centre; FDC, follicular dendritic cell; Immunoglobulin; PMA, phorbol-12-myristate-13-acetate; IgV, immunoglobulin variable region; FCS, foetal calf serum; Lymphocytes; TGF, transforming growth factor; CD25, IL-2 receptor alpha chain, TAC; 2ME, 2-mercaptoethanol; Culture; IFN, interferon; Ig, immunoglobulin; MN, mononuclear; SRBC, sheep red blood cells; mIg, membrane immunoglobulin; SAC, Staphylococcus Cowan 1 strain; NK, natural killer; CD40L, CD40 ligand, CD154; IL, interleukin; AET, amino-ethylisothiouronium; Ag, antigen; EBV, Epstein–Barr virus; Human; Cell culture; Immunoglobulins; Production; Review; Method; In vitro; Lymphocyte
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/S0022-1759(99)00244-6

The synthesis and secretion of immunoglobulin induced in cultured B-lineage cells is of interest for several reasons: (i) analysing the B-cell repertoire, (ii) recall of immunological activity retained in the circulating lymphocyte population, and (iii) study of factors needed for clonal expansion, immunoglobulin class switching, IgV-region mutation and maturation of cells to Ig secretion. Methods available are outlined and alternative procedures for cell separation and purification, helper cell provision and Ab/Ig assay systems are discussed. The aim is to provide practical guidance for those who intend to begin work in what is a vitally important, but experimentally difficult, area.There are a bewildering number of methods described in innumerable publications, old and new. The review provides a personal assessment of the present state of knowledge and prospects for improvements when all the new observations relating to cell–cell interactions and cytokines are integrated into existing technologies. The survey is chiefly concerned with physiologically based procedures, but artificial auxiliary methods are also briefly mentioned.